SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCSR2
Systematic nameYPR030W
AliasMRG19
DescriptionTranscription factor CSR2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K841        
2 MULTI(K63-linked) HECTNARSP5 UBP2 
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidenceUb-conjugate Confirmation
Methodautoradiography (in vitro)
Reference"The deubiquitinating enzyme Ubp2 modulates Rsp5-dependent Lys63-linked polyubiquitin conjugates in Saccharomyces cerevisiae."
Kee Y, Munoz W, Lyon N, Huibregtse JM.
J Biol Chem. 281(48):36724-31 (2006) [17028178]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ12734
SGD YPR030W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007047cell wall organization and biogenesisIGI:SGD.
Biological processGO:0006357regulation of transcription from RNA polyme...IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1121
Molecular weight124849

	---------+----------+----------+----------+----------+
MQSTVPIAIA SNGNKRDVVQ NVSAGDEGDI LQRLARNREM ISTSLSPQKS   50
SGFSGRRRSS SVRDALSSFF GTGNSPTSSM DDYSNLMNRN YSTASTAMCR   100
GNSFPSDVGT KAYNITGSYQ PDRHRNSVPY TTIDQLHTRQ DTGLRRESDP   150
VAAKQISSNN DIVRSFITHH ASNSTMFINR VLSDYLADRG FIKQTPLYNK   200
KSVLEISIAT SAESVFLPTT KSDETEYLSL IHGSLNQART QPVGSTNTAE   250
SDFLPSCPTM DTLNENNDLS LFPLHTQRTS PSNTARTGNA MDTSNSDRAS   300
PASNNNTTDA DSFVASGNNN PMNNNNSPAR NRHPNSHSRS LPNAWNSQMP   350
SFSFALIFSL NKSTTLSDIK VELTSNVRVV WFNGLPPTKN VNEECYNIGS   400
LDWTLNADNF NLFIPQGAKS PLDIVENHSN NRKLKVLQKL SMRKRRSFSN   450
KAVLRENILN NLNASNSTNK LNAGVYVFTI PIVLASRIPE SLYYPSARVS   500
YSLRLATKLK DEHTQLVASR PRSSSISSPQ KLRSYSCSDS YEYSQIDDTI   550
EGETYNNDKN STGKIAFPSS WLKSAKGRLK RNNSNGRSDN NGASSSGLAM   600
QHDSEDTINL QYPLNLVRTP PEISVTTANK PLYINKVWEN CLSYEISFAQ   650
KYVPLNGEIP ITIKVAPLVK SLSVKRIRVS CREKISYRSK DYQYDFDQLD   700
PLASDPCNPY HMRYLVRKKK DRSLPLFEVA SKCTSGPSIR EEVVTNTVDD   750
NLLAYTSSKE NNKDIPFSES FTVKTKLKFP KYCEVDATKA ASLPPYGIDL   800
FDPIKDPTQS ENTSNNGNVL GFLVGRPNRA SKTVHKIPQD KNHNEVNDTN   850
GNSNTSLQTS SNVPIQHYTR LNKPRRGLYL DSMHFKNIQC SHKLEIVLRV   900
SKTDSGSSKI IRHYEVIVDT PIYLISDLCN TSNIDLPTYD MATTESSKVL   950
PPTFEEATSV SASPRSSVSY YPDDISMQQL NLSRSTSLAN GYLSTLHPKT   1000
TAVSDSSNGA PIRDQQEQQA RPLRTEDYAL QMGNENNAYS NMDGLLSQDI   1050
FEQETAATLF KRDIVTMNFN NNIFTPRYSP RTFTNTDYNY NDNDNNDNDT   1100
EGPGPIIHPG PEPPRYDEIS S                                  1121

	* Ubiquitinated lysine is denoted as red color.