SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCSG2
Systematic nameYBR036C
AliasCLS2
DescriptionMannosyl phosphorylinositol ceramide synthase regulatory protein CSG2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP35206
SGD YBR036C
Gene Ontology (GO)
Cellular componentGO:0030176integral to endoplasmic reticulum membraneIDA:SGD.
Molecular functionGO:0030234enzyme regulator activityIMP:SGD.
Biological processGO:0006874calcium ion homeostasisIMP:SGD.
Biological processGO:0042493response to drugIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length410
Molecular weight45515

	---------+----------+----------+----------+----------+
MSTTLLWFSS VIGYVIQTKC LSNIQSKKEI SVGPNGTIAT PETNGDNGNS   50
SSLTFYLTFM YFASWLLWVP ASRLWEKMRP MFVSDSDSNR NSQFDNNNSG   100
SVTNEDVDTF SHVLDDPQPR IPAQQQKQKI ISVATFKYVA KLTVLALIMI   150
VADLTYNMAL SLSPAFDVAL MQNTAIFEIV TLLYGVCGIS RKNYVFRNFL   200
IMMNAVIGIL IISYTKATCD MLAGKLSVNP NTGELSDPFL FDRLKGALIC   250
GLGALIMGPF AVLWNRWFCS NISKNENSAV VLVKQSTHMA LIGIIGMVIL   300
LPFIPKFPSR ESVESISLFY NDKSFWFSLL GSIIFGSLPS LISILELNRK   350
APAEYLTTCN LGAIIFMGLA EWVCEPTQTT IVRWEVIGYI MLTVSLLVLS   400
VTLGEGKYHH                                                410