SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCPS1
Systematic nameYJL172W
AliasCPS
DescriptionCarboxypeptidase S

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5  Vacuolar Degradation
2   HECTNARSP5  Vacuolar Degradation
3         
4   HECTNARSP5  Vacuolar Degradation
5  UBC4RINGSPRFTUL1 DOA4 
6        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Multivesicular body sorting: ubiquitin ligase Rsp5 is required for the modification and sorting of carboxypeptidase S."
Katzmann DJ, Sarkar S, Chu T, Audhya A, Emr SD.
Mol Biol Cell. 15(2):468-80 (2004) [14657247]
[2]EvidenceHigh MW Product Confirmation
Methodfluorescence microscopy (GFP (delivery to the interior of the vacuole))
Western blot
Reference"The ubiquitin ligase Rsp5p is required for modification and sorting of membrane proteins into multivesicular bodies."
Morvan J, Froissard M, Haguenauer-Tsapis R, Urban-Grimal D.
Traffic. 5(5):383-92 (2004) [15086787]
[3]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[4]EvidenceHigh MW Product Confirmation
MethodWestern blot (in vitro)
Reference"Multiple interactions drive adaptor-mediated recruitment of the ubiquitin ligase rsp5 to membrane proteins in vivo and in vitro."
Sullivan JA, Lewis MJ, Nikko E, Pelham HR.
Mol Biol Cell. 18(7):2429-40 (2007) [17429078]
[5]EvidenceUb-conjugate Confirmation
MethodWestern Blot
Reference"A transmembrane ubiquitin ligase required to sort membrane proteins into multivesicular bodies."
Reggiori F, Pelham HR.
Nat Cell Biol. 4(2):117-23 (2002) [11788821]
[6]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP27614
SGD YJL172W
Gene Ontology (GO)
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyTAS:SGD.
Molecular functionGO:0004226Gly-X carboxypeptidase activityIMP:SGD.
Biological processGO:0006807nitrogen compound metabolic processIMP:SGD.
Biological processGO:0006508proteolysisIMP:SGD.
EC number 3.4.17.4

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length576
Molecular weight64597

	---------+----------+----------+----------+----------+
MIALPVEKAP RKSLWQRHRA FISGIVALII IGTFFLTSGL HPAPPHEAKR   50
PHHGKGPMHS PKCEKIEPLS PSFKHSVDTI LHDPAFRNSS IEKLSNAVRI   100
PTVVQDKNPN PADDPDFYKH FYELHDYFEK TFPNIHKHLK LEKVNELGLL   150
YTWEGSDPDL KPLLLMAHQD VVPVNNETLS SWKFPPFSGH YDPETDFVWG   200
RGSNDCKNLL IAEFEAIEQL LIDGFKPNRT IVMSLGFDEE ASGTLGAASL   250
ASFLHERYGD DGIYSIIDEG EGIMEVDKDV FVATPINAEK GYVDFEVSIL   300
GHGGHSSVPP DHTTIGIASE LITEFEANPF DYEFEFDNPI YGLLTCAAEH   350
SKSLSKDVKK TILGAPFCPR RKDKLVEYIS NQSHLRSLIR TTQAVDIING   400
GVKANALPET TRFLINHRIN LHSSVAEVFE RNIEYAKKIA EKYGYGLSKN   450
GDDYIIPETE LGHIDITLLR ELEPAPLSPS SGPVWDILAG TIQDVFENGV   500
LQNNEEFYVT TGLFSGNTDT KYYWNLSKNI YRFVGSIIDI DLLKTLHSVN   550
EHVDVPGHLS AIAFVYEYIV NVNEYA                             576