SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCPR4
Systematic nameYCR069W
AliasCYP4, SCC3
DescriptionPeptidyl-prolyl cis-trans isomerase CPR4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP25334
SGD YCR069W
Gene Ontology (GO)
EC number 5.2.1.8

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length318
Molecular weight35780

	---------+----------+----------+----------+----------+
MWLKSLLLCL YSLVLCQVHA APSSGKQITS KDVDLQKKYE PSPPATHRGI   50
ITIEYFDPVS KSMKEADLTF ELYGTVVPKT VNNFAMLAHG VKAVIEGKDP   100
NDIHTYSYRK TKINKVYPNK YIQGGVVAPD VGPFTVYGPK FDDENFYLKH   150
DRPERLAMAY FGPDSNTSEF IITTKADGNE ELDGKSVVFG QITSGLDQLM   200
DAIQYTETDE YGKPQHELRF LYFVLEILKI SNILDLHAAY TEKVEKFRNG   250
DVSVGSTLEN IFRNDKAYTP LTTSTGTTAY DLNHPISRAL MCLTVLGLCF   300
IAYKGMHEKP HTVSLRHK                                      318