SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCPR1
Systematic nameYDR155C
AliasCPH1, CYP1, SCC1
DescriptionPeptidyl-prolyl cis-trans isomerase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP14832
SGD YDR155C
Gene Ontology (GO)
Cellular componentGO:0000118histone deacetylase complexIPI:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0003755peptidyl-prolyl cis-trans isomerase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0019538protein metabolic processIMP:SGD.
Biological processGO:0030437sporulation (sensu Fungi)IMP:SGD.
EC number 5.2.1.8

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
145Phosphoserine.

Sequence Information
Sequence length162
Molecular weight17391

	---------+----------+----------+----------+----------+
MSQVYFDVEA DGQPIGRVVF KLYNDIVPKT AENFRALCTG EKGFGYAGSP   50
FHRVIPDFML QGGDFTAGNG TGGKSIYGGK FPDENFKKHH DRPGLLSMAN   100
AGPNTNGSQF FITTVPCPWL DGKHVVFGEV VDGYDIVKKV ESLGSPSGAT   150
KARIVVAKSG EL                                            162