SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCPA1
Systematic nameYOR303W
DescriptionCarbamoyl-phosphate synthase arginine-specific small chain

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
3        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP07258
SGD YOR303W
Gene Ontology (GO)
Molecular functionGO:0004088carbamoyl-phosphate synthase (glutamine-hyd...IMP:SGD.
Biological processGO:0006526arginine biosynthetic processTAS:SGD.
EC number 6.3.5.5

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length411
Molecular weight45362

	---------+----------+----------+----------+----------+
MSSAATKATF CIQNGPSFEG ISFGANKSVA GETVFTTSLV GYPESMTDPS   50
YRGQILVFTQ PLIGNYGVPS GEARDEYNLL KYFESPHIHV VGIVVAEYAY   100
QYSHWTAVES LAQWCQREGV AAITGVDTRE LVQYLREQGS SLGRITLADH   150
DPVPYVNPMK TNLVAQVTTK KPFHVSALPG KAKANVALID CGVKENIIRC   200
LVKRGANVTV FPYDYRIQDV ASEFDGIFLS NGPGNPELCQ ATISNVRELL   250
NNPVYDCIPI FGICLGHQLL ALASGASTHK LKYGNRAHNI PAMDLTTGQC   300
HITSQNHGYA VDPETLPKDQ WKPYFVNLND KSNEGMIHLQ RPIFSTQFHP   350
EAKGGPLDTA ILFDKFFDNI EKYQLQSQAK SSISLKVTYS TDKSRLQSIN   400
VTKLAKERVL F                                             411