SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCOT1
Systematic nameYOR316C
DescriptionCobalt uptake protein COT1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K301        
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS
Reference"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP32798
SGD YOR316C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyIDA:SGD.
Molecular functionGO:0015087cobalt ion transporter activityIMP:SGD.
Molecular functionGO:0005385zinc ion transporter activityIGI:SGD.
Biological processGO:0006824cobalt ion transportIMP:SGD.
Biological processGO:0006882zinc ion homeostasisIMP:SGD.
Biological processGO:0006829zinc ion transportIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
225Phosphoserine.

Sequence Information
Sequence length439
Molecular weight48155

	---------+----------+----------+----------+----------+
MKLGSKQVKI ISLLLLDTVF FGIEITTGYL SHSLALIADS FHMLNDIISL   50
VVALWAVNVA KNRNPDSTYT YGWKRAEILG ALINAVFLIA LCVSILIEAL   100
QRIIAPPVIE NPKFVLYVGV AGLISNTVGL FLFHDNDQEH GHGHGHSHGG   150
IFADHEMHMP SSHTHTHAHV DGIENTTPMD STDNISEIMP NAIVDSFMNE   200
NTRLLTPENA SKTPSYSTSS HTIASGGNYT EHNKRKRSLN MHGVFLHVLG   250
DALGNIGVML SAFFIWKTDY SWKYYTDPLV SLIITGIIFS SALPLSCKAS   300
KILLQATPST LSGDQVEGDL LKIPGIIAIH DFHIWNLTES IFIASLHIQL   350
DISPEQFTDL AKIVRSKLHR YGIHSATLQP EFITREVTST ERAGDSQGDH   400
LQNDPLSLRP KTYGTGISGS TCLIDDAANC NTADCLEDH               439

	* Ubiquitinated lysine is denoted as red color.