SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCOS4
Systematic nameYFL062W
DescriptionProtein COS4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K205        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
purification (6xHis-Myc-ubiquitin), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]

"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]

Cross References
Swiss-Prot accessionP43542
SGD YFL062W
Gene Ontology (GO)
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length379
Molecular weight45312

	---------+----------+----------+----------+----------+
MKENELKNEK SVDVLSFKQL ESQKIVLPQD LFRSSFTWFC YEIYKSLAFR   50
IWMLLWLPLS VWWKLSNNWI YPLMVSLLVL FWGPVFVLVI FRLSRKRSLS   100
KQLTQFCKEI TKSTPSSDPH DWEVVAANLN SYLYENKAWN IRYFFFNAMG   150
CQEAFRTTLL EPFSLKKDEA AKVKSFKDSV PYIEEALGVY FREVEKQWKL   200
FNSEKSWSPV GLEDAKLPKE AYRFKLTWFL KRISNIFMLI PFLNFLCCIY   250
VSRGMCLLLR TLYLGWILFM LVQGFQNIRV LIMSMEHKMQ FLSTIINEQE   300
SGANGWDEIA RKMNRYLFEK KVWKNEEFFF DGIDCEWFFS HFFYRVLSAK   350
KSMRALSLNV ELWPYIKEAQ LSCSEESLA                          379

	* Ubiquitinated lysine is denoted as red color.