SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCOG2
Systematic nameYGR120C
AliasSEC35
DescriptionConserved oligomeric Golgi complex subunit 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53271
SGD YGR120C
Gene Ontology (GO)
Cellular componentGO:0017119Golgi transport complexIPI:SGD.
Cellular componentGO:0000300peripheral to membrane of membrane fractionIDA:SGD.
Cellular componentGO:0005625soluble fractionIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006888ER to Golgi vesicle-mediated transportIGI:SGD.
Biological processGO:0000301retrograde transport, vesicle recycling wit...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length262
Molecular weight30325

	---------+----------+----------+----------+----------+
MDFLNDDELD LDLPVTAEIS KELFATEIEK YRESETNGTD VDNFDVDRFL   50
VQKNFHYLPL DSLIRDLSGL SQKMVQTLLE QIRSNYDDYL TFSNTYTDEE   100
NETLINLEKT QSDLQKFMTQ LDHLIKDDIS NTQEIIKDVL EYLKKLDEIY   150
GSLRNHSQLT EALSLGKRLS KSLHEMCGIE PLEEEICSGL IEQLYKLITA   200
SRRILESCAD SNSPYIHHLR NDYQDLLQEF QISLKILTEK CLENPSSLQN   250
LSLTLVSIIK TA                                            262