SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCNM67
Systematic nameYNL225C
DescriptionChaotic nuclear migration protein 67

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53865
SGD YNL225C
Gene Ontology (GO)
Cellular componentGO:0005824outer plaque of spindle pole bodyIDA:SGD.
Molecular functionGO:0005200structural constituent of cytoskeletonIPI:SGD.
Biological processGO:0007020microtubule nucleationIPI:SGD.
Biological processGO:0007052mitotic spindle organization and biogenesisIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
17Phosphoserine.
20Phosphoserine.
72Phosphoserine.
89Phosphoserine.
120Phosphoserine.
410Phosphotyrosine.
412Phosphothreonine.
413Phosphoserine.

Sequence Information
Sequence length581
Molecular weight67400

	---------+----------+----------+----------+----------+
MTDFDLMNFP FHERLDSPVS ENGEIKDGEP IPQNWLNENH VGKSILPLFV   50
NPEDVINCNF SNARDSYEEN KSPSMDQMNY ARNTSYQESP GLQERPKNEK   100
DKSPIGTDVH KKDVPNFIHS TPRENSSKHF TRANEQASAQ PTDEHTSPDI   150
SIEDCNGAKI FLQNSLSKED FRMLENVILG YQKKVIELGR DNLRQEERAN   200
SLQKELEAAT KSNDKTLDNK KKIEEQTVLI ENLTKDLSLN KEMLEKANDT   250
IQTKHTALLS LTDSLRKAEL FEIPIGILFF DLYDSEENSS KLDHILQEKY   300
PNIKGFLCAS QQEELSRISQ RFKNAKAEAE DLRNELENKK IEIQTMREKN   350
NTLIGTNKTL SKQNKILCDK FDKLTIDEKE ILKGCNEEIK IKLERLNERL   400
GSWEKSKEKY ETSLKDKEKM LADAEKKTNT LSKELDNLRS RFGNLEGNTS   450
ERITIKNILQ SRPDISAEEC NFLMVEQIDS ANLTTLQNTV KEIVLAVGIP   500
YPKLRRKIPL LAIKLKYENI MLSNFAQRLH RQVYSQEMNL KKFTDQAYYD   550
FMSTRRMDSI DHHLERCLDH LYDHILEKMV K                       581