SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameCNA2
Systematic nameYML057W
AliasCMP2
DescriptionSerine/threonine-protein phosphatase 2B catalytic subunit A2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP14747
SGD YML057W
Gene Ontology (GO)
Cellular componentGO:0005955calcineurin complexIPI:SGD.
Cellular componentGO:0005737cytoplasmTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000754adaptation to pheromone during conjugation ...IMP:SGD.
Biological processGO:0006873cell ion homeostasisIMP:SGD.
EC number 3.1.3.16

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
31Phosphothreonine.
520Phosphoserine (Potential).

Sequence Information
Sequence length604
Molecular weight68528

	---------+----------+----------+----------+----------+
MSSDAIRNTE QINAAIKIIE NKTERPQSST TPIDSKASTV AAANSTATET   50
SRDLTQYTLD DGRVVSTNRR IMNKVPAITS HVPTDEELFQ PNGIPRHEFL   100
RDHFKREGKL SAAQAARIVT LATELFSKEP NLISVPAPIT VCGDIHGQYF   150
DLLKLFEVGG DPATTSYLFL GDYVDRGSFS FECLIYLYSL KLNFNDHFWL   200
LRGNHECKHL TSYFTFKNEM LHKYNLDIYE KCCESFNNLP LAALMNGQYL   250
CVHGGISPEL NSLQDINNLN RFREIPSHGL MCDLLWADPI EEYDEVLDKD   300
LTEEDIVNSK TMVPHHGKMA PSRDMFVPNS VRGCSYAFTY RAACHFLQET   350
GLLSIIRAHE AQDAGYRMYK NTKTLGFPSL LTLFSAPNYL DTYNNKAAIL   400
KYENNVMNIR QFNMTPHPYW LPDFMDVFTW SLPFVGEKVT EMLVAILNIC   450
TEDELENDTP VIEELVGTDK KLPQAGKSEA TPQPATSASP KHASILDDEH   500
RRKALRNKIL AVAKVSRMYS VLREETNKVQ FLKDHNSGVL PRGALSNGVK   550
GLDEALSTFE RARKHDLINE KLPPSLDELK NENKKYYEKV WQKVHEHDAK   600
NDSK                                                     604