SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCLN2
Systematic nameYPL256C
DescriptionG1/S-specific cyclin CLN2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTICDC34      
2   RINGSCFGRR1   
3   RINGSCFGRR1   
4        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
Methodfluorography (in vitro)
Reference"Ubiquitination of the G1 cyclin Cln2p by a Cdc34p-dependent pathway."
Deshaies RJ, Chau V, Kirschner M.
EMBO J. 14(2):303-12 (1995) [7835341]
[2]EvidenceProtein Stability Assay
Methodfluorography
Reference"G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast."
Barral Y, Jentsch S, Mann C.
Genes Dev. 9(4):399-409 (1995) [7883165]
[3]EvidenceInteraction Assay
Methodwith substrate receptor
Reference"F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex."
Skowyra D, Craig KL, Tyers M, Elledge SJ, Harper JW.
Cell. 91(2):209-19 (1997) [9346238]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP20438
SGD YPL256C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000321re-entry into mitotic cell cycle after pher...IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length545
Molecular weight61697

	---------+----------+----------+----------+----------+
MASAEPRPRM GLVINAKPDY YPIELSNAEL LSHFEMLQEY HQEISTNVIA   50
QSCKFKPNPK LIDQQPEMNP VETRSNIITF LFELSVVTRV TNGIFFHSVR   100
LYDRYCSKRI VLRDQAKLVV ATCLWLAAKT WGGCNHIINN VVIPTGGRFY   150
GPNPRARIPR LSELVHYCGD GQVFDESMFL QMERHILDTL NWNIYEPMIN   200
DYVLNVDENC LMQYELYENQ VTYDKQCSEK RQSQLSQDSD ATVDERPYQN   250
EEEEEEDLKL KIKLINLKKF LIDVSAWQYD LLRYELFEVS HGIFSIINQF   300
TNQDHGPFLM TPMTSESKNG EILSTLMNGI VSIPNSLMEV YKTVNGVLPF   350
INQVKEYHLD LQRKLQIASN LNISRKLTIS TPSCSFENSN STSIPSPASS   400
SQSHTPMRNM SSLSDNSVFS RNMEQSSPIT PSMYQFGQQQ SNSICGSTVS   450
VNSLVNTNNK QRIYEQITGP NSNNATNDYI DLLNLNESNK ENQNPATAHY   500
LNGGPPKTSF INHGMFPSPT GTINSGKSSS ASSLISFGMG NTQVI        545