SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCLN1
Systematic nameYMR199W
DescriptionG1/S-specific cyclin CLN1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGSCFGRR1   
2  CDC34RINGSCFGRR1   
3 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
Methodfluorography
Reference"G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast."
Barral Y, Jentsch S, Mann C.
Genes Dev. 9(4):399-409 (1995) [7883165]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot (in vitro)
Reference"F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex."
Skowyra D, Craig KL, Tyers M, Elledge SJ, Harper JW.
Cell. 91(2):209-19 (1997) [9346238]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP20437
SGD YMR199W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length546
Molecular weight62050

	---------+----------+----------+----------+----------+
MNHSEVKTGL IVTAKQTYYP IELSNAELLT HYETIQEYHE EISQNVLVQS   50
SKTKPDIKLI DQQPEMNPHQ TREAIVTFLY QLSVMTRVSN GIFFHAVRFY   100
DRYCSKRVVL KDQAKLVVGT CLWLAAKTWG GCNHIINNVS IPTGGRFYGP   150
NPRARIPRLS ELVHYCGGSD LFDESMFIQM ERHILDTLNW DVYEPMINDY   200
ILNVDENCLI QYELYKNQLQ NNNSNGKEWS CKRKSQSSDD SDATVEEHIS   250
SSPQSTGLDG DTTTMDEDEE LNSKIKLINL KRFLIDLSCW QYNLLKFELY   300
EICNGMFSII NKFTNQDQGP FLSMPIGNDI NSNTQTQVFS IIINGIVNSP   350
PSLVEVYKEQ YGIVPFILQV KDYNLELQKK LQLASTIDLT RKIAVNSRYF   400
DQNASSSSVS SPSTYSSGTN YTPMRNFSAQ SDNSVFSTTN IDHSSPITPH   450
MYTFNQFKNE SACDSAISVS SLPNQTQNGN MPLSSNYQNM MLEERNKENR   500
IPNSSSAEIP QRAKFMTTGI FQNTGELTNR ASSISLSLRN HNSSQL       546