SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCLB5
Systematic nameYPR120C
DescriptionS-phase entry cyclin-5

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGAPCCDC20   
2   RINGAPCAMA1   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
Methodfluorescence microscopy
Reference"APC(Cdc20) promotes exit from mitosis by destroying the anaphase inhibitor Pds1 and cyclin Clb5."
Shirayama M, Toth A, Galova M, Nasmyth K.
Nature. 402(6758):203-7 (1999) [10647015]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Mitotic phosphorylation of the anaphase-promoting complex inhibitory subunit Mnd2 is necessary for efficient progression through meiosis i."
Torres MP, Borchers CH.
J Biol Chem. 282(24):17351-62 (2007) [17459880]

Cross References
Swiss-Prot accessionP30283
SGD YPR120C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Biological processGO:0000082G1/S transition of mitotic cell cycleIEP:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIEP:SGD.
Biological processGO:0045740positive regulation of DNA replicationIMP:SGD.
Biological processGO:0006279premeiotic DNA synthesisIGI:SGD.
Biological processGO:0007090regulation of S phase of mitotic cell cycleIMP:SGD.
Biological processGO:0051225spindle assemblyIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length435
Molecular weight50431

	---------+----------+----------+----------+----------+
MGENHDHEQS IKRNSMIYNE NERQLCNSNL KILQNKRALS KNDSSSKQQV   50
QDSKPRRALT DVPVNNNPLS QNKRIVAGSK AAKVRREENI RPIVSAVQKR   100
QIYNDRTAAE QEEEEEEEGE DDDAASIVNK KRRIDAEGVS EIVGWQDLDY   150
VEKDDTAMVA EYSAEIFAFL YRRELETLPS HNYLLDKTSK YYLRPSMRTI   200
LVDWLVEVHE KFQCYPETLF LSINLMDRFL AKNKVTMNKL QLLAVTSLFI   250
AAKFEEVNLP KLAEYAYITD GAASKNDIKN AEMFMLTSLE FNIGWPNPLN   300
FLRRISKADD YDPVNRNIGK FILEYAYCCH QFIHLPPSTV SAMAMYIARR   350
MTNRNKNELW NGTLQHYSGG IDPIHDEAFQ SLCIDLVKDI ASSKTHLDSL   400
ILKYKKPRYG SVYFQTFKWC TSEMHSNFQN LFNLK                   435