SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCLB2
Systematic nameYPR119W
DescriptionG2/mitotic-specific cyclin-2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGAPCCDH1   
2   RINGAPCCDH1   
3        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis."
Visintin R, Prinz S, Amon A.
Science. 278(5337):460-3 (1997) [9334304]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC."
Schwab M, Neutzner M, M?cker D, Seufert W.
EMBO J. 20(18):5165-75 (2001) [11566880]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP24869
SGD YPR119W
Gene Ontology (GO)
Cellular componentGO:0005935cellular bud neckIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0005816spindle pole bodyIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000086G2/M transition of mitotic cell cycleIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length491
Molecular weight56247

	---------+----------+----------+----------+----------+
MSNPIENTEN SQNTSSSRFL RNVQRLALNN VTNTTFQKSN ANNPALTNFK   50
STLNSVKKEG SRIPQFTRES VSRSTAAQEE KRTLKENGIQ LPKNNLLDDK   100
ENQDPSSQQF GALTSIKEGR AELPANISLQ ESSSAKEIIQ HDPLKGVGSS   150
TEVVHNSVEN EKLHPARSQL QVRNTESETD SGKKRPISTI VEQELPKKFK   200
VCDENGKEEY EWEDLDAEDV NDPFMVSEYV NDIFEYLHQL EVITLPKKED   250
LYQHRNIHQN RDILVNWLVK IHNKFGLLPE TLYLAINIMD RFLGKELVQL   300
DKLQLVGTSC LFIASKYEEV YSPSIKHFAS ETDGACTEDE IKEGEKFILK   350
TLKFNLNYPN PMNFLRRISK ADDYDIQSRT LAKFLLEISL VDFRFIGILP   400
SLCAAAAMFM SRKMLGKGKW DGNLIHYSGG YTKEELAPVC HMIMDYLVSP   450
IVHDEFHRKY QSRRFMKASI ISVQWALKVR KNGYDIMTLH E            491