SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCIT1
Systematic nameYNR001C
AliasGLU3, LYS6
DescriptionCitrate synthase, mitochondrial

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP00890
SGD YNR001C
Gene Ontology (GO)
Cellular componentGO:0005759mitochondrial matrixTAS:SGD.
Molecular functionGO:0004108citrate (Si)-synthase activityTAS:SGD.
Biological processGO:0006101citrate metabolic processTAS:SGD.
Biological processGO:0006537glutamate biosynthetic processTAS:SGD.
Biological processGO:0006099tricarboxylic acid cycleTAS:SGD.
EC number 2.3.3.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length479
Molecular weight53360

	---------+----------+----------+----------+----------+
MSAILSTTSK SFLSRGSTRQ CQNMQKALFA LLNARHYSSA SEQTLKERFA   50
EIIPAKAEEI KKFKKEHGKT VIGEVLLEQA YGGMRGIKGL VWEGSVLDPE   100
EGIRFRGRTI PEIQRELPKA EGSTEPLPEA LFWLLLTGEI PTDAQVKALS   150
ADLAARSEIP EHVIQLLDSL PKDLHPMAQF SIAVTALESE SKFAKAYAQG   200
VSKKEYWSYT FEDSLDLLGK LPVIASKIYR NVFKDGKITS TDPNADYGKN   250
LAQLLGYENK DFIDLMRLYL TIHSDHEGGN VSAHTTHLVG SALSSPYLSL   300
AAGLNGLAGP LHGRANQEVL EWLFKLREEV KGDYSKETIE KYLWDTLNAG   350
RVVPGYGHAV LRKTDPRYTA QREFALKHFP DYELFKLVST IYEVAPGVLT   400
KHGKTKNPWP NVDSHSGVLL QYYGLTEASF YTVLFGVARA IGVLPQLIID   450
RAVGAPIERP KSFSTEKYKE LVKKIESKN                          479