SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCHS1
Systematic nameYNL192W
DescriptionChitin synthase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP08004
SGD YNL192W
Gene Ontology (GO)
Cellular componentGO:0045009chitosomeIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0004100chitin synthase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007114cell buddingTAS:SGD.
Biological processGO:0007109cytokinesis, completion of separationIMP:SGD.
EC number 2.4.1.16

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
34Phosphoserine.
270Phosphoserine.
299Phosphoserine.
305Phosphoserine.
318Phosphoserine.
328Phosphothreonine.

Sequence Information
Sequence length1131
Molecular weight129919

	---------+----------+----------+----------+----------+
MSDQNNRSRN EYHSNRKNEP SYELQNAHSG LFHSSNEELT NRNQRYTNQN   50
ASMGSFTPVQ SLQFPEQSQQ TNMLYNGDDG NNNTINDNER DIYGGFVNHH   100
RQRPPPATAE YNDVFNTNSQ QLPSEHQYNN VPSYPLPSIN VIQTTPELIH   150
NGSQTMATPI ERPFFNENDY YYNNRNSRTS PSIASSSDGY ADQEARPILE   200
QPNNNMNSGN IPQYHDQPFG YNNGYHGLQA KDYYDDPEGG YIDQRGDDYQ   250
INSYLGRNGE MVDPYDYENS LRHMTPMERR EYLHDDSRPV NDGKEELDSV   300
KSGYSHRDLG EYDKDDFSRD DEYDDLNTID KLQFQANGVP ASSSVSSIGS   350
KESDIIVSND NLTANRALKR SGTEIRKFKL WNGNFVFDSP ISKTLLDQYA   400
TTTENANTLP NEFKFMRYQA VTCEPNQLAE KNFTVRQLKY LTPRETELML   450
VVTMYNEDHI LLGRTLKGIM DNVKYMVKKK NSSTWGPDAW KKIVVCIISD   500
GRSKINERSL ALLSSLGCYQ DGFAKDEINE KKVAMHVYEH TTMINITNIS   550
ESEVSLECNQ GTVPIQLLFC LKEQNQKKIN SHRWAFEGFA ELLRPNIVTL   600
LDAGTMPGKD SIYQLWREFR NPNVGGACGE IRTDLGKRFV KLLNPLVASQ   650
NFEYKMSNIL DKTTESNFGF ITVLPGAFSA YRFEAVRGQP LQKYFYGEIM   700
ENEGFHFFSS NMYLAEDRIL CFEVVTKKNC NWILKYCRSS YASTDVPERV   750
PEFILQRRRW LNGSFFASVY SFCHFYRVWS SGHNIGRKLL LTVEFFYLFF   800
NTLISWFSLS SFFLFFRILT VSIALAYHSA FNVLSVIFLW LYGICTLSTF   850
ILSLGNKPKS TEKFYVLTCV IFAVMMIYMI FCSIFMSVKS FQNILKNDTI   900
SFEGLITTEA FRDIVISLGS TYCLYLISSI IYLQPWHMLT SFIQYILLSP   950
SYINVLNIYA FCNVHDLSWG TKGAMANPLG KINTTEDGTF KMEVLVSSSE   1000
IQANYDKYLK VLNDFDPKSE SRPTEPSYDE KKTGYYANVR SLVIIFWVIT   1050
NFIIVAVVLE TGGIADYIAM KSISTDDTLE TAKKAEIPLM TSKASIYFNV   1100
ILWLVALSAL IRFIGCSIYM IVRFFKKVTF R                       1131