SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCHC1
Systematic nameYGL206C
DescriptionClathrin heavy chain

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP22137
SGD YGL206C
Gene Ontology (GO)
Cellular componentGO:0030125clathrin vesicle coatTAS:SGD.
Molecular functionGO:0005198structural molecule activityTAS:SGD.
Biological processGO:0006897endocytosisIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1653
Molecular weight187234

	---------+----------+----------+----------+----------+
MSDLPIEFTE LVDLMSLGIS PQFLDFRSTT FESDHFVTVR ETKDGTNSVA   50
IVDLAKGNEV TRKNMGGDSA IMHPSQMVIS VRANGTIVQI FNLETKSKLK   100
SFTLDEPVIF WRWLSETTLG FVTARSILTS NVFDGNVNAK PQLLTLRHAN   150
LNNTQIINFV ANKNLDWFAV VGILQENGRI AGRIQLFSKQ RNISQAIDGH   200
VAIFTNILLE GNGSTPVQVF VTGNRNATTG AGELRIIEID HDASLPSQYQ   250
KETTDIFFPP DATNDFPIAV QVSEKYGIIY LLTKYGFIHL YELETGTNLF   300
VNRITAESVF TAAPYNHENG IACINKKGQV LAVEISTSQI VPYILNKLSN   350
VALALIVATR GGLPGADDLF QKQFESLLLQ NDYQNAAKVA ASSTSLRNQN   400
TINRLKNIQA PPGAISPILL YFSTLLDKGK LNKEETIELA RPVLQQDRKQ   450
LFEKWLKEDK LECSEELGDI VKPFDTTLAL ACYLRAGAHA KVISCLAELQ   500
QFEKIIPYCQ KVGYQPNFLV LISSLIRSSP DRASEFAVSL LQNPETASQI   550
DIEKIADLFF SQNHIQQGTS LLLDALKGDT PDQGHLQTRV LEVNLLHAPQ   600
VADAILGNNI FSHYDKPTIA SLSEKAGLYQ RALENYTDIK DIKRCVVHTN   650
ALPIDWLVGY FGKLNVEQSL ACLKALMDNN IQANIQTVVQ VATKFSDLIG   700
PSTLIKLFED YNATEGLYYY LASLVNLTED KDVVYKYIEA AAKMKQYREI   750
ERIVKDNNVY DPERVKNFLK DANLEDQLPL VIVCDRFDFV HEMILYLYKS   800
QNLKFIETYV QQVNPSKTAQ VVGALLDMDC DEAFIQSLLQ SVLGQVPINE   850
LTTEVEKRNR LKILLPFLEQ SLSQGIQDQA VYNALAKIYI DSNNSPEKFL   900
KENDQYDTLD VGHYCEKRDP YLAYIAYEKG QNDDDLIRIT NENSMYKYQA   950
RYLLERSDLD LWNKVLNQEN IHRRQLIDSV ISVGIPELTD PEPVSLTVQA   1000
FMTNGLKLEL IELLEKIILE PSPFNENVAL QGLLLLSAIK YEPTKVSSYI   1050
EKLDNYDADE IAPLCIEHDL KEEAFEIYDK HEMYGKALKV LIEDIMSLDR   1100
AASYADKINT PELWSQIGTA QLDGLRIPDA IESYIKAEDP SNYENVIDIA   1150
EQAGKYEELI PFLLMARKTL KEPKIDGALI LAYAELNKIH EIENLLAGSN   1200
VANLDHVGDK LFENKEYKAA RLCYSAVSNY SKLASTLVYL GDYQAAVDTA   1250
RKASNIKVWK LVNDACIEKK EFKLAQICGL NLIVHAEELD ELVERYESNG   1300
YFEELISLFE AGLGLERAHM GMFTELAILY SKYEPDKTFE HLKLFWSRIN   1350
IPKVIRAVEQ AHLWSELVFL YAHYDEWDNA ALTLIEKSTK DLDHAYFKEV   1400
VVKVSNLEIY YKAINFYVKF HPSLLVDLLT SLTPRLDIPR TVKIFSKSDN   1450
LPLIKPFLIN VLPKNNSVVN QAYHDLMIEE EDYKALQDAV DSYDKFDQLG   1500
LASRLESHKL IFFKKIGALL YRRNKKWAKS LSILKEEKLW KDAIETAAIS   1550
QDPKVVEALL TYFVETGNRE GFVALLYAAY NLVRIEFVLE ISWMNSLEDY   1600
IKPFEISIKK EQNDSIKKIT EELAKKSGSN EEHKDGQPLM LMNSAMNVQP   1650
TGF                                                      1653