SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC60
Systematic nameYPL160W
DescriptionLeucyl-tRNA synthetase, cytoplasmic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP26637
SGD YPL160W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0004823leucine-tRNA ligase activityIDA:SGD.
Biological processGO:0006429leucyl-tRNA aminoacylationIDA:SGD.
EC number 6.1.1.4

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
142Phosphothreonine.

Sequence Information
Sequence length1090
Molecular weight124142

	---------+----------+----------+----------+----------+
MSSGLVLENT ARRDALIAIE KKYQKIWAEE HQFEIDAPSI EDEPITMDSE   50
ELHRTYPKFM SSMAYPYMNG VMHAGHCFTL SKVEFSIGFE RMNGKRALFP   100
LGFHCTGMPI LACADKLKRE AELFGKNFDN VPAEEEEIKE ETPAEKDHED   150
VTKFKAKKSK AAAKKGRGKY QFEIMLQLGI PREEIIKFAD AKYWLTYFPP   200
LCESDCTSLG ARIDWRRSFV TTDANPYYDA FIRWQMNKLK AAGKIKFGER   250
YTIYSEKDGQ ACMDHDRQSG EGVTPQEYIG VKIEALEFAD DAAKIIDSSS   300
DLDKSKKFYF VAATLRPETM YGQTCCFVSP TIEYGIFDAG DSYFITTERA   350
FKNMSYQKLT PKRGFYKPIV TVPGKAFIGT KIHAPQSVYP ELRILPMETV   400
IATKGTGVVT CVPSNSPDDY ITTKDLLHKP EYYGIKPEWI DHEIVPIMHT   450
EKYGDLTAKA IVEEKKIQSP KDKNLLAEAK KIAYKEDYYT GTMIYGPYKG   500
EKVEQAKNKV KADMIAAGEA FVYNEPESQV MSRSGDDCIV SLEDQWYVDY   550
GEESWKKQAI ECLEGMQLFA PEVKNAFEGV LDWLKNWAVC RTYGLGTRLP   600
WDEKYLVESL SDSTIYQSFY TIAHLLFKDY YGNEIGPLGI SADQMTDEVF   650
DYIFQHQDDV KNTNIPLPAL QKLRREFEYF YPLDVSISGK DLIPNHLTFF   700
IYTHVALFPK KFWPKGIRAN GHLMLNNSKM SKSTGNFMTL EQTVEKFGAD   750
AARIAFADAG DTVEDANFDE SNANAAILRL FNLKEWAEEI TKESNLRTGE   800
ITDFFDIAFE HEMNALIEKT YEQYALTNYK NALKYGLFDF QAARDYYREA   850
SGVMHKDLIA RYIETQALLL APIAPHFAEY IYREVLGNQT SVQNAKFPRA   900
SKPVDKGVLA ALDYLRNLQR SIREGEGQAL KKKKGKSAEI DASKPVKLTL   950
LISESFPEWQ SQCVEIVRKL FSEQTLDDNK KVREHIEPKE MKRAMPFISL   1000
LKQRLANEKP EDVFERELQF SEIDTVKAAA RNVKKAAQAL KIAEFSAISF   1050
PYGAKTGKDI FTGEEVEIPP VTKIVENAVP GNPGVVFQNI               1090