SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC6
Systematic nameYJL194W
DescriptionCell division control protein 6

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1  CDC34RINGSCFCDC4   
2  CDC34RINGSCFCDC4   
3  CDC34RINGSCFCDC4   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast."
Drury LS, Perkins G, Diffley JF.
EMBO J. 16(19):5966-76 (1997) [9312054]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"The Cdc6 protein is ubiquitinated in vivo for proteolysis in Saccharomyces cerevisiae."
Sanchez M, Calzada A, Bueno A.
J Biol Chem. 274(13):9092-7 (1999) [10085159]
[3]EvidenceHigh MW Product Confirmation
Methodautoradiography (in vitro)
Reference"Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis."
Elsasser S, Chi Y, Yang P, Campbell JL.
Mol Biol Cell. 10(10):3263-77 (1999) [10512865]

Cross References
Swiss-Prot accessionP09119
SGD YJL194W
Gene Ontology (GO)
Cellular componentGO:0005656pre-replicative complexTAS:SGD.
Molecular functionGO:0003689DNA clamp loader activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006267pre-replicative complex assemblyTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length513
Molecular weight58037

	---------+----------+----------+----------+----------+
MSAIPITPTK RIRRNLFDDA PATPPRPLKR KKLQFTDVTP ESSPEKLQFG   50
SQSIFLRTKA LLQKSSELVN LNSSDGALPA RTAEYEQVMN FLAKAISEHR   100
SDSLYITGPP GTGKTAQLDM IIRQKFQSLP LSLSTPRSKD VLRHTNPNLQ   150
NLSWFELPDG RLESVAVTSI NCISLGEPSS IFQKIFDSFQ DLNGPTLQIK   200
NMQHLQKFLE PYHKKTTFVV VLDEMDRLLH ANTSETQSVR TILELFLLAK   250
LPTVSFVLIG MANSLDMKDR FLSRLNLDRG LLPQTIVFQP YTAEQMYEIV   300
IQKMSSLPTI IFQPMAIKFA AKKCAGNTGD LRKLFDVLRG SIEIYELEKR   350
FLLSPTRGSL NSAQVPLTPT TSPVKKSYPE PQGKIGLNYI AKVFSKFVNN   400
NSTRTRIAKL NIQQKLILCT IIQSLKLNSD ATIDESFDHY IKAITKTDTL   450
APLQRNEFLE ICTILETCGL VSIKKTKCKG KTKRFVDKID VDLDMREFYD   500
EMTKISILKP FLH                                           513