SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC54
Systematic nameYPR019W
AliasHCD21
DescriptionCell division control protein 54

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP30665
SGD YPR019W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0042555MCM complexIDA:SGD.
Cellular componentGO:0005656pre-replicative complexIDA:SGD.
Molecular functionGO:0004003ATP-dependent DNA helicase activityTAS:SGD.
Molecular functionGO:0003682chromatin bindingTAS:SGD.
Biological processGO:0006270DNA replication initiationTAS:SGD.
Biological processGO:0006268DNA unwinding during replicationTAS:SGD.
Biological processGO:0006267pre-replicative complex assemblyIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
69Phosphoserine.

Sequence Information
Sequence length933
Molecular weight105003

	---------+----------+----------+----------+----------+
MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN   50
NSQNLSQGEG NIRAAIGSSP LNFPSSSQRQ NSDVFQSQGR QGRIRSSASA   100
SGRSRYHSDL RSDRALPTSS SSLGRNGQNR VHMRRNDIHT SDLSSPRRIV   150
DFDTRSGVNT LDTSSSSAPP SEASEPLRII WGTNVSIQEC TTNFRNFLMS   200
FKYKFRKILD EREEFINNTT DEELYYIKQL NEMRELGTSN LNLDARNLLA   250
YKQTEDLYHQ LLNYPQEVIS IMDQTIKDCM VSLIVDNNLD YDLDEIETKF   300
YKVRPYNVGS CKGMRELNPN DIDKLINLKG LVLRSTPVIP DMKVAFFKCN   350
VCDHTMAVEI DRGVIQEPAR CERIDCNEPN SMSLIHNRCS FADKQVIKLQ   400
ETPDFVPDGQ TPHSISLCVY DELVDSCRAG DRIEVTGTFR SIPIRANSRQ   450
RVLKSLYKTY VDVVHVKKVS DKRLDVDTST IEQELMQNKV DHNEVEEVRQ   500
ITDQDLAKIR EVAAREDLYS LLARSIAPSI YELEDVKKGI LLQLFGGTNK   550
TFTKGGRYRG DINILLCGDP STSKSQILQY VHKITPRGVY TSGKGSSAVG   600
LTAYITRDVD TKQLVLESGA LVLSDGGVCC IDEFDKMSDS TRSVLHEVME   650
QQTISIAKAG IITTLNARSS ILASANPIGS RYNPNLPVTE NIDLPPPLLS   700
RFDLVYLVLD KVDEKNDREL AKHLTNLYLE DKPEHISQDD VLPVEFLTMY   750
ISYAKEHIHP IITEAAKTEL VRAYVGMRKM GDDSRSDEKR ITATTRQLES   800
MIRLAEAHAK MKLKNVVELE DVQEAVRLIR SAIKDYATDP KTGKIDMNLV   850
QTGKSVIQRK LQEDLSREIM NVLKDQASDS MSFNELIKQI NEHSQDRVES   900
SDIQEALSRL QQEDKVIVLG EGVRRSVRLN NRV                     933