SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC53
Systematic nameYDL132W
DescriptionCell division control protein 53

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ12018
SGD YDL132W
Gene Ontology (GO)
Cellular componentGO:0019005SCF ubiquitin ligase complexIDA:SGD.
Molecular functionGO:0003688DNA replication origin bindingIPI:SGD.
Molecular functionGO:0030674protein binding, bridgingIMP:SGD.
Biological processGO:0000082G1/S transition of mitotic cell cycleIMP:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIGI:SGD.
Biological processGO:0042787protein ubiquitination during ubiquitin-dep...IDA:SGD.
Biological processGO:0031146SCF-dependent proteasomal ubiquitin-depende...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length815
Molecular weight93944

	---------+----------+----------+----------+----------+
MSETLPRSDD LEATWNFIEP GINQILGNEK NQASTSKRVY KILSPTMYME   50
VYTAIYNYCV NKSRSSGHFS TDSRTGQSTI LVGSEIYEKL KNYLKNYILN   100
FKQSNSETFL QFYVKRWKRF TIGAIFLNHA FDYMNRYWVQ KERSDGKRHI   150
FDVNTLCLMT WKEVMFDPSK DVLINELLDQ VTLGREGQII QRSNISTAIK   200
SLVALGIDPQ DLKKLNLNVY IQVFEKPFLK KTQEYYTQYT NDYLEKHSVT   250
EYIFEAHEII KREEKAMTIY WDDHTKKPLS MALNKVLITD HIEKLENEFV   300
VLLDARDIEK ITSLYALIRR DFTLIPRMAS VFENYVKKTG ENEISSLLAM   350
HKHNIMKNEN ANPKKLALMT AHSLSPKDYI KKLLEVHDIF SKIFNESFPD   400
DIPLAKALDN ACGAFININE FALPAGSPKS ATSKTSEMLA KYSDILLKKA   450
TKPEVASDMS DEDIITIFKY LTDKDAFETH YRRLFAKRLI HGTSTSAEDE   500
ENIIQRLQAA NSMEYTGKIT KMFQDIRLSK ILEDDFAVAL KNEPDYSKAK   550
YPDLQPFVLA ENMWPFSYQE VEFKLPKELV PSHEKLKESY SQKHNGRILK   600
WLWPLCRGEL KADIGKPGRM PFNFTVTLFQ MAILLLYNDA DVLTLENIQE   650
GTSLTIQHIA AAMVPFIKFK LIQQVPPGLD ALVKPETQFK LSRPYKALKT   700
NINFASGVKN DILQSLSGGG HDNHGNKLGN KRLTEDERIE KELNTERQIF   750
LEACIVRIMK AKRNLPHTTL VNECIAQSHQ RFNAKVSMVK RAIDSLIQKG   800
YLQRGDDGES YAYLA                                         815