SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC5
Systematic nameYMR001C
AliasMSD2, PKX2
DescriptionCell cycle serine/threonine-protein kinase CDC5/MSD2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGAPCCDH1   
2   RINGAPCCDH1   
3 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae."
Charles JF, Jaspersen SL, Tinker-Kulberg RL, Hwang L, Szidon A, Morgan DO.
Curr Biol. 8(9):497-507 (1998) [9560342]
[2]EvidenceInteraction Assay
Methodwith substrate receptor
Reference"Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC."
Schwab M, Neutzner M, M?cker D, Seufert W.
EMBO J. 20(18):5165-75 (2001) [11566880]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP32562
SGD YMR001C
Gene Ontology (GO)
Cellular componentGO:0005935cellular bud neckIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0000922spindle poleIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Molecular functionGO:0004672protein kinase activityIDA:SGD.
Biological processGO:0006261DNA-dependent DNA replicationIDA:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
EC number 2.7.11.21

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
419Phosphoserine.

Sequence Information
Sequence length705
Molecular weight81031

	---------+----------+----------+----------+----------+
MSLGPLKAIN DKQLNTRSKL VHTPIKGNTA DLVGKENHFK QTKRLDPNND   50
HHHQPAQKKK REKLSALCKT PPSLIKTRGK DYHRGHFLGE GGFARCFQIK   100
DDSGEIFAAK TVAKASIKSE KTRKKLLSEI QIHKSMSHPN IVQFIDCFED   150
DSNVYILLEI CPNGSLMELL KRRKVLTEPE VRFFTTQICG AIKYMHSRRV   200
IHRDLKLGNI FFDSNYNLKI GDFGLAAVLA NESERKYTIC GTPNYIAPEV   250
LMGKHSGHSF EVDIWSLGVM LYALLIGKPP FQARDVNTIY ERIKCRDFSF   300
PRDKPISDEG KILIRDILSL DPIERPSLTE IMDYVWFRGT FPPSIPSTVM   350
SEAPNFEDIP EEQSLVNFKD CMEKSLLLES MSSDKIQRQK RDYISSIKSS   400
IDKLEEYHQN RPFLPHSLSP GGTKQKYKEV VDIEAQRRLN DLAREARIRR   450
AQQAVLRKEL IATSTNVIKS EISLRILASE CHLTLNGIVE AEAQYKMGGL   500
PKSRLPKIKH PMIVTKWVDY SNKHGFSYQL STEDIGVLFN NGTTVLRLAD   550
AEEFWYISYD DREGWVASHY LLSEKPRELS RHLEVVDFFA KYMKANLSRV   600
STFGREEYHK DDVFLRRYTR YKPFVMFELS DGTFQFNFKD HHKMAISDGG   650
KLVTYISPSH ESTTYPLVEV LKYGEIPGYP ESNFREKLTL IKEGLKQKST   700
IVTVD                                                    705