SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC47
Systematic nameYBR202W
AliasMCM7
DescriptionDNA replication licensing factor CDC47

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38132
SGD YBR202W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0042555MCM complexIDA:SGD.
Cellular componentGO:0005656pre-replicative complexIDA:SGD.
Molecular functionGO:0005524ATP bindingIDA:SGD.
Molecular functionGO:0004003ATP-dependent DNA helicase activityTAS:SGD.
Molecular functionGO:0003682chromatin bindingTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006270DNA replication initiationTAS:SGD.
Biological processGO:0006268DNA unwinding during replicationTAS:SGD.
Biological processGO:0006267pre-replicative complex assemblyIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
811Phosphothreonine.
819Phosphoserine.

Sequence Information
Sequence length845
Molecular weight94875

	---------+----------+----------+----------+----------+
MSAALPSIQL PVDYNNLFNE ITDFLVTFKQ DTLSSDATRN ENEDENLDAE   50
NIEQHLLEKG PKYMAMLQKV ANRELNSVII DLDDILQYQN EKFLQGTQAD   100
DLVSAIQQNA NHFTELFCRA IDNNMPLPTK EIDYKDDVLD VILNQRRLRN   150
ERMLSDRTNE IRSENLMDTT MDPPSSMNDA LREVVEDETE LFPPNLTRRY   200
FLYFKPLSQN CARRYRKKAI SSKPLSVRQI KGDFLGQLIT VRGIITRVSD   250
VKPAVEVIAY TCDQCGYEVF QEVNSRTFTP LSECTSEECS QNQTKGQLFM   300
STRASKFSAF QECKIQELSQ QVPVGHIPRS LNIHVNGTLV RSLSPGDIVD   350
VTGIFLPAPY TGFKALKAGL LTETYLEAQF VRQHKKKFAS FSLTSDVEER   400
VMELITSGDV YNRLAKSIAP EIYGNLDVKK ALLLLLVGGV DKRVGDGMKI   450
RGDINVCLMG DPGVAKSQLL KAICKISPRG VYTTGKGSSG VGLTAAVMKD   500
PVTDEMILEG GALVLADNGI CCIDEFDKMD ESDRTAIHEV MEQQTISISK   550
AVINTNPGAR TSILAAANPL YGRINPRLSP LDNINLPAAL LSRFDILFLM   600
LDIPSRDDDE KLAEHVTYVH MHNKQPDLDF TPVEPSKMRE YIAYAKTKRP   650
VMSEAVNDYV VQAYIRLRQD SKREMDSKFS FGQATPRTLL GIIRLSQALA   700
KLRLADMVDI DDVEEALRLV RVSKESLYQE TNKSKEDESP TTKIFTIIKK   750
MLQETGKNTL SYENIVKTVR LRGFTMLQLS NCIQEYSYLN VWHLINEGNT   800
LKFVDDGTMD TDQEDSLVST PKLAPQTTAS ANVSAQDSDI DLQDA        845