SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC4
Systematic nameYFL009W
DescriptionCell division control protein 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 AUTOCDC34RINGSCFCDC4  Proteasomal Degradation
2 AUTOCDC34RINGSCFCDC4  Proteasomal Degradation
3 AUTOCDC34RINGSCFCDC4   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Ubiquitination and degradation of the substrate recognition subunits of SCF ubiquitin-protein ligases."
Zhou P, Howley PM.
Mol Cell. 2(5):571-80 (1998) [9844630]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanism."
Galan JM, Peter M.
Proc Natl Acad Sci U S A. 96(16):9124-9 (1999) [10430906]
[3]EvidenceHigh MW Product Confirmation
MethodWestern blot (in vitro)
Reference"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
Kus BM, Caldon CE, Andorn-Broza R, Edwards AM.
Proteins. 54(3):455-67 (2004) [14747994]

Cross References
Swiss-Prot accessionP07834
SGD YFL009W
Gene Ontology (GO)
Cellular componentGO:0016363nuclear matrixIDA:SGD.
Cellular componentGO:0043224nuclear SCF ubiquitin ligase complexIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000082G1/S transition of mitotic cell cycleIMP:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIGI:SGD.
Biological processGO:0007126meiosisIMP:SGD.
Biological processGO:0042787protein ubiquitination during ubiquitin-dep...IDA:SGD.
Biological processGO:0031146SCF-dependent proteasomal ubiquitin-depende...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length779
Molecular weight86090

	---------+----------+----------+----------+----------+
MGSFPLAEFP LRDIPVPYSY RVSGGIASSG SVTALVTAAG THRNSSTAKT   50
VETEDGEEDI DEYQRKRAAG SGESTPERSD FKRVKHDNHK TLHPVNLQNT   100
GAASVDNDGL HNLTDISNDA EKLLMSVDDG SAAPSTLSVN MGVASHNVAA   150
PTTVNAATIT GSDVSNNVNS ATINNPMEEG ALPLSPTASS PGTTTPLAKT   200
TKTINNNNNI ADLIESKDSI ISPEYLSDEI FSAINNNLPH AYFKNLLFRL   250
VANMDRSELS DLGTLIKDNL KRDLITSLPF EISLKIFNYL QFEDIINSLG   300
VSQNWNKIIR KSTSLWKKLL ISENFVSPKG FNSLNLKLSQ KYPKLSQQDR   350
LRLSFLENIF ILKNWYNPKF VPQRTTLRGH MTSVITCLQF EDNYVITGAD   400
DKMIRVYDSI NKKFLLQLSG HDGGVWALKY AHGGILVSGS TDRTVRVWDI   450
KKGCCTHVFK GHNSTVRCLD IVEYKNIKYI VTGSRDNTLH VWKLPKESSV   500
PDHGEEHDYP LVFHTPEENP YFVGVLRGHM ASVRTVSGHG NIVVSGSYDN   550
TLIVWDVAQM KCLYILSGHT DRIYSTIYDH ERKRCISASM DTTIRIWDLE   600
NIWNNGECSY ATNSASPCAK ILGAMYTLQG HTALVGLLRL SDKFLVSAAA   650
DGSIRGWDAN DYSRKFSYHH TNLSAITTFY VSDNILVSGS ENQFNIYNLR   700
SGKLVHANIL KDADQIWSVN FKGKTLVAAV EKDGQSFLEI LDFSKASKIN   750
YVSNPVNSSS SSLESISTSL GLTRTTIIP                          779