SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC34
Systematic nameYDR054C
AliasDNA6, UBC3
DescriptionUbiquitin-conjugating enzyme E2-34 kDa

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP14682
SGD YDR054C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0019005SCF ubiquitin ligase complexIDA:SGD.
Molecular functionGO:0042803protein homodimerization activityIDA:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0000082G1/S transition of mitotic cell cycleTAS:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIGI:SGD.
Biological processGO:0051865protein autoubiquitinationIDA:SGD.
Biological processGO:0000209protein polyubiquitinationIDA:SGD.
Biological processGO:0042787protein ubiquitination during ubiquitin-dep...IDA:SGD.
Biological processGO:0031146SCF-dependent proteasomal ubiquitin-depende...IDA:SGD.
EC number 6.3.2.19

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length295
Molecular weight34065

	---------+----------+----------+----------+----------+
MSSRKSTASS LLLRQYRELT DPKKAIPSFH IELEDDSNIF TWNIGVMVLN   50
EDSIYHGGFF KAQMRFPEDF PFSPPQFRFT PAIYHPNVYR DGRLCISILH   100
QSGDPMTDEP DAETWSPVQT VESVLISIVS LLEDPNINSP ANVDAAVDYR   150
KNPEQYKQRV KMEVERSKQD IPKGFIMPTS ESAYISQSKL DEPESNKDMA   200
DNFWYDSDLD DDENGSVILQ DDDYDDGNNH IPFEDDDVYN YNDNDDDDER   250
IEFEDDDDDD DDSIDNDSVM DRKQPHKAED ESEDVEDVER VSKKI        295