SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameCDC3
Systematic nameYLR314C
DescriptionCell division control protein 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI HECTNARSP5   
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodluminescent assay (biotin-ubiquitin, GST-substrate)
Reference"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5."
Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, Lee T, Chan D, Wolting C, Edwards A, Bosse R, Rotin D.
J Biol Chem. 280(33):29470-8 (2005) [15955809]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32457
SGD YLR314C
Gene Ontology (GO)
Cellular componentGO:0000144cellular bud neck septin ringTAS:SGD.
Cellular componentGO:0001400mating projection baseIDA:SGD.
Cellular componentGO:0005628prospore membraneTAS:SGD.
Cellular componentGO:0005619spore wall (sensu Fungi)TAS:SGD.
Molecular functionGO:0005545phosphatidylinositol bindingIDA:SGD.
Molecular functionGO:0005200structural constituent of cytoskeletonTAS:SGD.
Biological processGO:0007120axial cellular bud site selectionTAS:SGD.
Biological processGO:0007047cell wall organization and biogenesisTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
9Phosphoserine.
73Phosphothreonine.
175Phosphoserine.

Sequence Information
Sequence length520
Molecular weight60039

	---------+----------+----------+----------+----------+
MSLKEEQVSI KQDPEQEERQ HDQFNDVQIK QESQDHDGVD SQYTNGTQND   50
DSERFEAAES DVKVEPGLGM GITSSQSEKG QVLPDQPEIK FIRRQINGYV   100
GFANLPKQWH RRSIKNGFSF NLLCVGPDGI GKTTLMKTLF NNDDIEANLV   150
KDYEEELAND QEEEEGQGEG HENQSQEQRH KVKIKSYESV IEENGVKLNL   200
NVIDTEGFGD FLNNDQKSWD PIIKEIDSRF DQYLDAENKI NRHSINDKRI   250
HACLYFIEPT GHYLKPLDLK FMQSVYEKCN LIPVIAKSDI LTDEEILSFK   300
KTIMNQLIQS NIELFKPPIY SNDDAENSHL SERLFSSLPY AVIGSNDIVE   350
NYSGNQVRGR SYPWGVIEVD NDNHSDFNLL KNLLIKQFME ELKERTSKIL   400
YENYRSSKLA KLGIKQDNSV FKEFDPISKQ LEEKTLHEAK LAKLEIEMKT   450
VFQQKVSEKE KKLQKSETEL FARHKEMKEK LTKQLKALED KKKQLELSIN   500
SASPNVNHSP VPTKKKGFLR                                     520