SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC20
Systematic nameYGL116W
DescriptionAPC/C activator protein CDC20

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGAPCCDH1   
2 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p."
Huang JN, Park I, Ellingson E, Littlepage LE, Pellman D.
J Cell Biol. 154(1):85-94 (2001) [11448992]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP26309
SGD YGL116W
Gene Ontology (GO)
Cellular componentGO:0005680anaphase-promoting complexTAS:SGD.
Molecular functionGO:0008047enzyme activator activityTAS:SGD.
Biological processGO:0007092anaphase-promoting complex activation durin...IMP:SGD.
Biological processGO:0008054cyclin catabolic processTAS:SGD.
Biological processGO:0000070mitotic sister chromatid segregationTAS:SGD.
Biological processGO:0000022mitotic spindle elongationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length610
Molecular weight67360

	---------+----------+----------+----------+----------+
MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS   50
SSLNIRNSKR PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD   100
KATFSAYSSR SYPTIGSESV VSQTSLSQPT TSREVDEQFT VAADRYIPIL   150
QGASQNKVDP ETLHEALPPP NASPISHLRA QTKIVFKQNV AEACGLDMNK   200
RILQYMPEPP KCSSLRQKSY IMKKRTHYSY QQEQKIPDLI KLRKINTNPE   250
RILDAPGFQD DFYLNLLSWS KKNVLAIALD TALYLWNATT GDVSLLTDFE   300
NTTICSVTWS DDDCHISIGK EDGNTEIWDV ETMSLIRTMR SGLGVRIGSL   350
SWLDTLIATG SRSGEIQIND VRIKQHIVST WAEHTGEVCG LSYKSDGLQL   400
ASGGNDNTVM IWDTRTSLPQ FSKKTHTAAV KALSWCPYSP NILASGGGQT   450
DKHIHFWNSI TGARVGSINT GSQVSSLHWG QSHTSTNGGM MNKEIVATGG   500
NPENAISVYN YETKFKVAEV VHAHEARICC SQLSPDGTTL ATVGGDENLK   550
FYKIFDPRCT GRSREDGLMD GMLGLIGKEG CRTNDKENRS KNSSEIHTRR   600
PSSTSQYLIR                                                610