SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCDC16
Systematic nameYKL022C
DescriptionAnaphase-promoting complex subunit CDC16

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP09798
SGD YKL022C
Gene Ontology (GO)
Cellular componentGO:0005680anaphase-promoting complexTAS:SGD.
Molecular functionGO:0005515protein bindingTAS:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityTAS:SGD.
Biological processGO:0031145anaphase-promoting complex-dependent protea...IDA:SGD.
Biological processGO:0008054cyclin catabolic processTAS:SGD.
Biological processGO:0007091mitotic metaphase/anaphase transitionTAS:SGD.
Biological processGO:0000070mitotic sister chromatid segregationTAS:SGD.
Biological processGO:0000022mitotic spindle elongationTAS:SGD.
Biological processGO:0016567protein ubiquitinationIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length840
Molecular weight94992

	---------+----------+----------+----------+----------+
MKFCLYCCHC YIVICGKATH YYKSSKATSN LKSSNRVLMR NPMSPSEQHS   50
QHNSTLAASP FVSNVSAART QQSLPTDAQN DRLQQPWNRT NTATSPYQSL   100
ANSPLIQKLQ ANIMTPHQPS ANSNSNSNSI TGNVVNDNNL LASMSKNSMF   150
GSTIPSTLRK VSLQREYKDS VDGVVRDEDN DEDVHNNGDA AANANNDRES   200
KLGHNGPLTT TTLTTTTTAT QLDVSELSAI ERLRLWRFDA LMQHMYRTAE   250
YIADKVYNIS NDPDDAFWLG QVYYNNNQYV RAVELITRNN LDGVNILCRY   300
LLGLSFVKLQ RFDDALDVIG EYNPFSEDPS TTAANTMSNN GNNSNTSQPV   350
TDGGIKMESS LCFLRGKIYF AQNNFNKARD AFREAILVDI KNFEAFEMLL   400
SKNLLTPQEE WDLFDSLDFK EFGEDKEIMK NLYKINLSKY INTEDITKSN   450
EILAKDYKLA DNVDVVRSKV DICYTQCKFN ECLELCETVL ENDEFNTNIL   500
PAYIGCLYEL SNKNKLFLLS HRLAETFPKS AITWFSVATY YMSLDRISEA   550
QKYYSKSSIL DPSFAAAWLG FAHTYALEGE QDQALTAYST ASRFFPGMHL   600
PKLFLGMQFM AMNSLNLAES YFVLAYDICP NDPLVLNEMG VMYFKKNEFV   650
KAKKYLKKAL EVVKDLDPSS RTTISIQLNL GHTYRKLNEN EIAIKCFRCV   700
LEKNDKNSEI HCSLGYLYLK TKKLQKAIDH LHKSLYLKPN NSSATALLKN   750
ALELNVTLSL DASHPLIDKS NLMSQASKDK ASLNKKRSSL TYDPVNMAKR   800
LRTQKEIFDQ NNKALRKGGH DSKTGSNNAD DDFDADMELE               840