SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCCT8
Systematic nameYJL008C
DescriptionT-complex protein 1 subunit theta

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K15        
2        Proteasomal Degradation
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP47079
SGD YJL008C
Gene Ontology (GO)
Cellular componentGO:0005832chaperonin-containing T-complexIPI:SGD.
Cellular componentGO:0005856cytoskeletonTAS:SGD.
Molecular functionGO:0051082unfolded protein bindingTAS:SGD.
Biological processGO:0007010cytoskeleton organization and biogenesisTAS:SGD.
Biological processGO:0006457protein foldingTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length568
Molecular weight61662

	---------+----------+----------+----------+----------+
MSLRLPQNPN AGLFKQGYNS YSNADGQIIK SIAAIRELHQ MCLTSMGPCG   50
RNKIIVNHLG KIIITNDAAT MLRELDIVHP AVKVLVMATE QQKIDMGDGT   100
NLVMILAGEL LNVSEKLISM GLSAVEIIQG YNMARKFTLK ELDEMVVGEI   150
TDKNDKNELL KMIKPVISSK KYGSEDILSE LVSEAVSHVL PVAQQAGEIP   200
YFNVDSIRVV KIMGGSLSNS TVIKGMVFNR EPEGHVKSLS EDKKHKVAVF   250
TCPLDIANTE TKGTVLLHNA QEMLDFSKGE EKQIDAMMKE IADMGVECIV   300
AGAGVGELAL HYLNRYGILV LKVPSKFELR RLCRVCGATP LPRLGAPTPE   350
ELGLVETVKT MEIGGDRVTV FKQEQGEISR TSTIILRGAT QNNLDDIERA   400
IDDGVAAVKG LMKPSGGKLL PGAGATEIEL ISRITKYGER TPGLLQLAIK   450
QFAVAFEVVP RTLAETAGLD VNEVLPNLYA AHNVTEPGAV KTDHLYKGVD   500
IDGESDEGVK DIREENIYDM LATKKFAINV ATEAATTVLS IDQIIMAKKA   550
GGPRAPQGPR PGNWDQED                                      568

	* Ubiquitinated lysine is denoted as red color.