SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCCT4
Systematic nameYDL143W
AliasANC2, TCP4
DescriptionT-complex protein 1 subunit delta

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP39078
SGD YDL143W
Gene Ontology (GO)
Cellular componentGO:0005832chaperonin-containing T-complexIPI:SGD.
Cellular componentGO:0005856cytoskeletonTAS:SGD.
Molecular functionGO:0051082unfolded protein bindingTAS:SGD.
Biological processGO:0007010cytoskeleton organization and biogenesisTAS:SGD.
Biological processGO:0006457protein foldingTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length528
Molecular weight57604

	---------+----------+----------+----------+----------+
MSAKVPSNAT FKNKEKPQEV RKANIIAARS VADAIRTSLG PKGMDKMIKT   50
SRGEIIISND GHTILKQMAI LHPVARMLVE VSAAQDSEAG DGTTSVVILT   100
GALLGAAERL LNKGIHPTII ADSFQSAAKR SVDILLEMCH KVSLSDREQL   150
VRAASTSLSS KIVSQYSSFL APLAVDSVLK ISDENSKNVD LNDIRLVKKV   200
GGTIDDTEMI DGVVLTQTAI KSAGGPTRKE KAKIGLIQFQ ISPPKPDTEN   250
NIIVNDYRQM DKILKEERAY LLNICKKIKK AKCNVLLIQK SILRDAVNDL   300
ALHFLSKLNI MVVKDIEREE IEFLSKGLGC KPIADIELFT EDRLGSADLV   350
EEIDSDGSKI VRVTGIRNNN ARPTVSVVIR GANNMIIDET ERSLHDALCV   400
IRCLVKERGL IAGGGAPEIE ISRRLSKEAR SMEGVQAFIW QEFASALEVI   450
PTTLAENAGL NSIKVVTELR SKHENGELND GISVRRSGTT NTYEEHILQP   500
VLVSTSAITL ASECVKSILR IDDIAFSR                           528