SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCBF5
Systematic nameYLR175W
DescriptionH/ACA ribonucleoprotein complex subunit 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP33322
SGD YLR175W
Gene Ontology (GO)
Cellular componentGO:0031429box H/ACA snoRNP complexIPI:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004730pseudouridylate synthase activityIMP:SGD.
Biological processGO:000636535S primary transcript processingIMP:UniProtKB.
Biological processGO:0031118rRNA pseudouridine synthesisIMP:SGD.
EC number 5.4.99.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
47Phosphoserine.
378Phosphothreonine.
399Phosphoserine, by ATM or ATR.
405Phosphothreonine.

Sequence Information
Sequence length483
Molecular weight54705

	---------+----------+----------+----------+----------+
MSKEDFVIKP EAAGASTDTS EWPLLLKNFD KLLVRSGHYT PIPAGSSPLK   50
RDLKSYISSG VINLDKPSNP SSHEVVAWIK RILRCEKTGH SGTLDPKVTG   100
CLIVCIDRAT RLVKSQQGAG KEYVCIVRLH DALKDEKDLG RSLENLTGAL   150
FQRPPLISAV KRQLRVRTIY ESNLIEFDNK RNLGVFWASC EAGTYMRTLC   200
VHLGMLLGVG GHMQELRRVR SGALSENDNM VTLHDVMDAQ WVYDNTRDES   250
YLRSIIQPLE TLLVGYKRIV VKDSAVNAVC YGAKLMIPGL LRYEEGIELY   300
DEIVLITTKG EAIAVAIAQM STVDLASCDH GVVASVKRCI MERDLYPRRW   350
GLGPVAQKKK QMKADGKLDK YGRVNENTPE QWKKEYVPLD NAEQSTSSSQ   400
ETKETEEEPK KAKEDSLIKE VETEKEEVKE DDSKKEKKEK KDKKEKKEKK   450
EKKDKKEKKE KKEKKRKSED GDSEEKKSKK SKK                     483