SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCAT8
Systematic nameYMR280C
AliasMSP8
DescriptionRegulatory protein CAT8

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP39113
SGD YMR280C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIC:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003704specific RNA polymerase II transcription fa...IDA:SGD.
Biological processGO:0045722positive regulation of gluconeogenesisIMP:SGD.
Biological processGO:0045944positive regulation of transcription from R...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1433
Molecular weight160485

	---------+----------+----------+----------+----------+
MANNNSDRQG LEPRVIRTLG SQALSGPSIS NRTSSSEANP HFSKNVKEAM   50
IKTASPTPLS TPIYRIAQAC DRCRSKKTRC DGKRPQCSQC AAVGFECRIS   100
DKLLRKAYPK GYTESLEERV RELEAENKRL LALCDIKEQQ ISLVSQSRPQ   150
TSTDNTINGN FKHDLKDAPL NLSSTNIYLL NQTVNKQLQN GKMDGDNSGS   200
AMSPLGAPPP PPHKDHLCDG VSCTNHLHVK PTSTSLNDPT AISFEQDEAP   250
GLPAVKALKS MTTHQRSTQL ATLVSLSIPR STEEILFIPQ LLTRIRQIFG   300
FNSKQCLYTV SLLSSLKNRL PAPRLLAPST STKLKEKDED KKLDDDSAFV   350
KRFQSTNLSE FVDLKKFLIS LKFNINSFSK QSEKPANDQD DELLSLTEIK   400
ELLHLFFKFW SNQVPILNND HFLIYFNNFV EVVKHLSTEN LETNNTTKST   450
VTTNHEIFAL KLLMMLQMGL LVKIKMEKIK YTVPKNPKAK YARLMAYYHQ   500
LSLIIPKNPY FLNMSTTSLP SLQLLSLASF YYLNVGDISA IYGVRGRIVS   550
MAQQLRLHRC PSAVLSVHSN PVLQKFEQSE RRLLFWAIYY VDVFASLQLG   600
VPRLLKDFDI ECALPISDVE YKDQLSMENE KADKKAKKIQ LQGQVSSFSL   650
QIIRFAKILG NILDSIFKRG MMDERITSEV ALVHENALDN WRNQLPEMYY   700
FQITVNGTVN LDEIRATNQR NTETKFDKKD IILFEKKILL LFYFLAKSMI   750
HLPVIATKPL PKNVDNATKK KQSMFNNDSK GATNQDHMIL DVDMTSPAIR   800
TSSSYIILQQ ATNATLTIFQ AINSMYLPLP LNVSRTLIRF SLLCARGSLE   850
YTKGGALFLD NKNLLLDTIK DIENDRLLDL PGIASWHTLK LFDMSINLLL   900
KAPNVKVERL DKFLEKKLNY YNRLMGLPPA TTTSLKPLFG SQSKNSLENR   950
QRTPNVKREN PEHEYLYGND SNNNNNSEAG HSPMTNTTNG NKRLKYEKDA   1000
KRNAKDGGIS KGENAHNFQN DTKKNMSTSN LFPFSFSNTD LTALFTHPEG   1050
PNCTNTNNGN VDVCNRASTD ATDANIENLS FLNMAPFLQT GNSNIGQNTI   1100
ENKPMHMDAI FSLPSNLDLM KDNMDSKPEQ LEPVIKQNPE NSKNNQFHQK   1150
GKSTNMEKNN LSFNNKSNYS LTKLMRLLNN DNSFSNISIN NFLYQNDQNS   1200
ASADPGTNKK AVTNAGANFK PPSTGSNTSQ GSILGSTKHG MDNCDFNDLG   1250
NFNNFMTNVN YSGVDYDYIV DASLGLAPLL VDTPDISNTN TTSTTSNRSK   1300
NSIILDTTFN DDLDRSRMNA REVLNPTDSI LSQGMVSSVS TRNTSNQRSL   1350
SSGNDSKGDS SSQENSKSAT GNQLDTPSTL FQMRRTSSGP SASHRGPRRP   1400
QKNRYNTDRS KSSGGGSSNT DNVSDLFQWQ NAK                     1433