SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCAK1
Systematic nameYFL029C
AliasCIV1
DescriptionSerine/threonine-protein kinase CAK1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP43568
SGD YFL029C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0019912cyclin-dependent protein kinase activating ...IDA:SGD.
Biological processGO:0000080G1 phase of mitotic cell cycleIMP:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIMP:SGD.
Biological processGO:0007126meiosisIGI:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
EC number 2.7.11.22

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length368
Molecular weight42186

	---------+----------+----------+----------+----------+
MKLDSIDITH CQLVKSTRTA RIYRSDTYAI KCLALDFDIP PHNAKFEVSI   50
LNKLGNKCKH ILPLLESKAT DNNDLLLLFP FEEMNLYEFM QMHYKRDRRK   100
KNPYYDLLNP SIPIVADPPV QKYTNQLDVN RYSLSFFRQM VEGIAFLHEN   150
KIIHRDIKPQ NIMLTNNTST VSPKLYIIDF GISYDMANNS QTSAEPMDSK   200
VTDISTGIYK APEVLFGVKC YDGGVDVWSL LIIISQWFQR ETSRMGHVPA   250
MIDDGSDDMN SDGSDFRLIC SIFEKLGIPS IQKWEEVAQH GSVDAFVGMF   300
GADGDGKYVL DQEKDVQISI VERNMPRLDE IADVKVKQKF INCILGMVSF   350
SPNERWSCQR ILQELEKP                                      368