SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBUL1
Systematic nameYMR275C
AliasDAG1, RDS1, SMM2
DescriptionUbiquitin ligase-binding protein BUL1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI HECTNARSP5   
2   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodluminescent assay (biotin-ubiquitin, GST-substrate)
Reference"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5."
Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, Lee T, Chan D, Wolting C, Edwards A, Bosse R, Rotin D.
J Biol Chem. 280(33):29470-8 (2005) [15955809]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]

Cross References
Swiss-Prot accessionP48524
SGD YMR275C
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIPI:SGD.
Cellular componentGO:0000151ubiquitin ligase complexIDA:SGD.
Molecular functionGO:0043130ubiquitin bindingTAS:SGD.
Biological processGO:0000001mitochondrion inheritanceIGI:SGD.
Biological processGO:0006513protein monoubiquitinationIMP:SGD.
Biological processGO:0000209protein polyubiquitinationIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
58Phosphoserine.
70Phosphoserine.
107Phosphothreonine.
111Phosphothreonine.

Sequence Information
Sequence length976
Molecular weight109174

	---------+----------+----------+----------+----------+
MAKDLNDSGF PPKRKPLLRP QRSDFTANSS TTMNVNANTR GRGRQKQEGG   50
KGSSRSPSLH SPKSWIRSAS ATGILGLRRP ELAHSHSHAP STGTPAGGNR   100
SPLRRSTANA TPVETGRSLT DGDINNVVDV LPSFEMYNTL HRHIPQGNVD   150
PDRHDFPPSY QEANNSTATG AAGSSADLSH QSLSTDALGA TRSSSTSNLE   200
NLIPLRTEHH SIAAHQSTAV DEDSLDIPPI LDDLNDTDNI FIDKLYTLPK   250
MSTPIEITIK TTKHAPIPHV KPEEESILKE YTSGDLIHGF ITIENKSQAN   300
LKFEMFYVTL ESYISIIDKV KSKRTIKRFL RMVDLSASWS YSKIALGSGV   350
DFIPADVDYD GSVFGLNNSR VLEPGVKYKK FFIFKLPLQL LDVTCKQEHF   400
SHCLLPPSFG IDKYRNNCKY SGIKVNRVLG CGHLGTKGSP ILTNDMSDDN   450
LSINYTIDAR IVGKDQKASK LYIMKEREYN LRVIPFGFDA NVVGERTTMS   500
QLNDITKLVQ ERLDALRKIF QRLEKKEPIT NRDIHGADLS GTIDDSIESD   550
SQEILQRKLD QLHIKNRNNY LVNYNDLKLG HDLDNGRSGN SGHNTDTSRA   600
WGPFVESELK YKLKNKSNSS SFLNFSHFLN SSSSSMSSSS NAGKNNHDLT   650
GNKERTGLIL VKAKIPKQGL PYWAPSLLRK TNVFESKSKH DQENWVRLSE   700
LIPEDVKKPL EKLDLQLTCI ESDNSLPHDP PEIQSITTEL ICITAKSDNS   750
IPIKLNSELL MNKEKLTSIK ALYDDFHSKI CEYETKFNKN FLELNELYNM   800
NRGDRRPKEL KFTDFITSQL FNDIESICNL KVSVHNLSNI FKKQVSTLKQ   850
HSKHALSEDS ISHTGNGSSS SPSSASLTPV TSSSKSSLFL PSGSSSTSLK   900
FTDQIVHKWV RIAPLQYKRD INVNLEFNKD IKETLIPSFE SCLCCRFYCV   950
RVMIKFENHL GVAKIDIPIS VRQVTK                             976