SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBUD4
Systematic nameYJR092W
DescriptionBud site selection protein 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP47136
SGD YJR092W
Gene Ontology (GO)
Cellular componentGO:0000142cellular bud neck contractile ringTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007120axial cellular bud site selectionTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
91Phosphoserine.
96Phosphoserine.
119Phosphoserine.
149Phosphoserine.
167Phosphoserine.
353Phosphoserine.
502Phosphoserine.
745Phosphoserine.
996Phosphoserine.
997Phosphoserine.

Sequence Information
Sequence length1447
Molecular weight164486

	---------+----------+----------+----------+----------+
MHDAESTVDS LLKEIDNEME QTKSNITQNG SEDTPHNWKL PLQEIGDDTM   50
EMLVKHNTRS NATENSRGRS PSKMSTISNE SLNLGLLRVN SELEESPAAV   100
HQERIKNSVA NGALGHANSP KVLNNLKNMA QDIDKLARDE EKPVKLSSSP   150
LKFTLKSTQP LLSYPESPIH RSSIEIETNY DDEDEEEEDA YTCLTQSPQI   200
LHSPSRIPIT NAVSINKLNL DFTLNPNESD KSLVSDTSVD STGRELDTKT   250
IPELPFCMSS TPEMTPVDEK CNLPSKLLNT SNNSHSDSRS PTASVEDLNI   300
STNLPGADSS QNNPVTTDAD ALIENDVVRD LQQNMEHIDD AFDEKKVLDE   350
GCSNEPVTFL GENDTRSIVY SNKGTNANVQ EFSQEDSLAH SEPKFKDLNA   400
TSDDVWNEDK ETDANISTST KSEESYIADY KVTRQEDWDT KKLHQESEHA   450
NEQPAIIPQK DSSEETFTEL NNESEFQRNF KDGEEYRIVQ HEESLYGQRT   500
KSPEENIING SEIGVDHGEA AEVNEPLAKT SAEEHDLSSS CEDQSVSEAR   550
NKDRIEEKEV ETKDENIETE KDESEYHKVE ENEEPEHVPL LPPLPRWEEI   600
QFNEPFIDEN DTSNDSIDLT RSMKPSDYIS IWHIQEEEIK SNSPESIANS   650
QFSQQSSITT ASTVDSKKDN GSTSFKFKPR IVSRSRIYNP KSRVSSLNYY   700
DNEDYILSNS EWNALDPMRR NTLISKRIQD NIRTQKGHAP LIRPSIMKLN   750
GEDSGFQNHF LEVEQPQEHE NIPLSTHLSE QDITTNVGLD EQKLPTNTQD   800
EAEISIREIE SAGDITFNRG DLLSLSFDEE LGQDFANFLD ALDHDSTSFN   850
HGPDDSSSFQ RDSSKKSFNS LWESSYELKP PPSIRKQPIA PDVLQKLLES   900
DTKDDADLEK IREERITEPR TGLGIGMLKT PVKDVSIALA ASIKGYEASF   950
SDTDSRPEGM NNSDAITLNM FDDFEEDKMT PSTPVRSISP IKRHVSSPFK   1000
VVKAGNKQEN NEINIKAEEE IEPMTQQETD GLKQDIPPLL AQTKDNVEAK   1050
EETITQLEEP QDVEQEFPDM GTLYLSIKAI STLALYGTKS HRATYAIVFD   1100
NGENVVQTPW ESLPYDGNIR INKEFELPID FKGKAETSSA SSERDSYKKC   1150
VITLKCKYEK PRHELVEIVD KVPVGKSFFG KTKYKFEKKY VQKKPKQDEW   1200
DYLFAQDGSF ARCEIEINEE FLKNVAFNTS HMHYNMINKW SRIADKIHGS   1250
KRLYELPRKA PHKVASLDVE ACFLERTSAF EQFPKQFSLV NKIVSKYKLQ   1300
QNIYKEGYLL QDGGDLKGKI ENRFFKLHGS QLSGYHEISR KAKIDINLLK   1350
VTKVLRNEDI QADNGGQRNF TDWVLFNECF QLVFDDGERI TFNAECSNEE   1400
KSDWYNKLQE VVELNVFHQP WVKKYCEKLA EEEKTRTTGH NLKQDFN      1447