SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBUD3
Systematic nameYCL014W
DescriptionBud site selection protein 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP25558
SGD YCL014W
Gene Ontology (GO)
Cellular componentGO:0000142cellular bud neck contractile ringTAS:SGD.
Biological processGO:0007120axial cellular bud site selectionTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
7Phosphoserine.
794Phosphoserine.
1075Phosphoserine.
1110Phosphoserine.
1111Phosphoserine.
1157Phosphothreonine.
1160Phosphoserine.
1183Phosphoserine.
1366Phosphoserine.
1412Phosphoserine.
1566Phosphothreonine.
1614Phosphoserine.

Sequence Information
Sequence length1636
Molecular weight184719

	---------+----------+----------+----------+----------+
MEKDLSSLYS EKKDKENDET LFNIKLSKSV VETTPLNGHS LFDDDKSLSD   50
WTDNVFTQSV FYHGSDDLIW GKFFVCVYKS PNSNKLNAII FDKLGTSCFE   100
SVDISSNSQY YPAIENLSPS DQESNVKKCI AVILLQRYPL LSPSDLSQIL   150
SNKSENCDYD PPYAGDLASS CQLITAVPPE DLGKRFFTSG LLQNRFVSST   200
LLDVIYENNE STIELNNRLV FHLGEQLEQL FNPVTEYSPE QTEYGYKAPE   250
DELPTESDDD LVKAICNELL QLQTNFTFNL VEFLQKFLIA LRVRVLNEEI   300
NGLSTTKLNR LFPPTIDEVT RINCIFLDSL KTAIPYGSLE VLKACSITIP   350
YFYKAYTRHE AATKNFSKDI KLFIRHFSNV IPEREVYTEM KIESIIKGPQ   400
EKLLKLKLII ERLWKSKKWR PKNQEMAKKC YNNIIDVIDS FGKLDSPLHS   450
YSTRVFTPSG KILTELAKCW PVELQYKWLK RRVVGVYDVV DLNDENKRNL   500
LVIFSDYVVF INILEAESYY TSDGSNRPLI SDILMNSLIN EVPLPSKIPK   550
LKVERHCYID EVLVSILDKS TLRFDRLKGK DSFSMVCKLS SAFISSSSVA   600
DLITKARILE KDTAFHLFKA SRSHFTLYST AHELCAYDSE KIKSKFALFL   650
NIPPSKEILE VNNLHLAFFA RFCSNDGRDN IVILDVLTKH DDKHIEVTSD   700
NIVFTIINQL AIEIPICFSS LNSSMAKDLL CVNENLIKNL EHQLEEVKHP   750
STDEHRAVNS KLSGASDFDA THEKKRSYGT ITTFRSYTSD LKDSPSGDNS   800
NVTKETKEIL PVKPTKKSSK KPREIQKKTK TNASKAEHIE KKKPNKGKGF   850
FGVLKNVFGS KSKSKPSPVQ RVPKKISQRH PKSPVKKPMT SEKKSSPKRA   900
VVSSPKIKKK STSFSTKESQ TAKSSLRAVE FKSDDLIGKP PDVGNGAHPQ   950
ENTRISSVVR DTKYVSYNPS QPVTENTSNE KNVEPKADQS TKQDNISNFA   1000
DVEVSASSYP EKLDAETDDQ IIGKATNSSS VHGNKELPDL AEVTTANRVS   1050
TTSAGDQRID TQSEFLRAAD VENLSDDDEH RQNESRVFND DLFGDFIPKH   1100
YRNKQENINS SSNLFPEGKV PQEKGVSNEN TNISLKTNED ASTLTQKLSP   1150
QASKVLTENS NELKDTNNEG KDAKDIKLGD DYSDKETAKE ITKPKNFVEG   1200
ITERKEIFPT IPRLAPPASK INFQRSPSYI ELFQGMRVVL DKHDAHYNWK   1250
RLASQVSLSE GLKVNTEEDA AIINKSQDDA KAERMTQISE VIEYEMQQPI   1300
PTYLPKAHLD DSGIEKSDDK FFEIEEELKE ELKGSKTGNE DVGNNNPSNS   1350
IPKIEKPPAF KVIRTSPVRI IGRTFEDTRK YENGSPSDIS FTYDTHNNDE   1400
PDKRLMELKF PSQDEIPDDR FYTPAEEPTA EFPVEELPNT PRSINVTTSN   1450
NKSTDDKLSS GNIDQKPTEL LDDLEFSSFN IAFGNTSMST DNMKISSDLS   1500
SNKTVLGNAQ KVQESPSGPL IYVLPQSSTK HEKEGFLRKK QKDEPIWVSP   1550
SKIDFADLSR RTKALTPERN TVPLKNNDSR KYKYTGEGSI GNMTNMLLTK   1600
DASYAYLKDF VALSDDEDED GKQNCAVGGP EKLKFY                  1636