SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBSP1
Systematic nameYPR171W
DescriptionProtein BSP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ06604
SGD YPR171W
Gene Ontology (GO)
Cellular componentGO:0030479actin cortical patchIDA:SGD.
Cellular componentGO:0005935cellular bud neckIDA:SGD.
Cellular componentGO:0005934cellular bud tipIDA:SGD.
Cellular componentGO:0005624membrane fractionIDA:SGD.
Molecular functionGO:0030674protein binding, bridgingIDA:SGD.
Biological processGO:0048228actin cortical patch distributionIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
39Phosphoserine.
46Phosphoserine.
309Phosphoserine.
320Phosphoserine.
535Phosphothreonine.
539Phosphothreonine.

Sequence Information
Sequence length576
Molecular weight64666

	---------+----------+----------+----------+----------+
MTKYERDPEL VNFLSKVEDL NSKRYSNIPS SKPAGEALSP VRSHNSGEYR   50
RADMMTGKNV EGCDNLAYRS AYNYEMTFSP KKTHYSLSEL NLERITPRPD   100
LEGSASQKEK KFLISEEDYL LLQKLKASQT YNDSNADKNL PSFEKGPRMP   150
SRGRPRPREK EIITIQYDFE LPGRADIPSS SSSSSPPPLP TRRDHIKITD   200
GNEEKPLLPT RPNKAEVTES PSSRSIKPDA VVPERVKPAP PVSRSTKPAS   250
FLSSLEDNKL TKAKSYNSEM ETPKTTVKSS HIDYLDSIQL KPTTLSPTMK   300
NKPKPTPPSP PAKRIPRSES FIKSMLNSNL TTTSKPSLPE KPQKLRNANL   350
AAHKTKPSIP PKKVELNIVL PELRPVETSP TKQNFENSID LPKLRSSNRN   400
IKKEEEDSIP EAIKGIQNLK KTKQQKPAIP QKKSFLTNNS KNTTLKNGDD   450
INKLNDEIEA LSLRNNLKKR PPTAPQRKIS LPEALRKVEL MKKSKTEPVL   500
ESSNELSINA KLDAIIASRN LRASNTLPEL SGVNTNIATS DKYTTSRDET   550
VKETKPLVHP NKNRTRGPRR KLPTRV                             576