SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBRO1
Systematic nameYPL084W
AliasASI6, LPF2, NPI3, VPS31
DescriptionVacuolar-sorting protein BRO1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP48582
SGD YPL084W
Gene Ontology (GO)
Cellular componentGO:0005768endosomeIDA:SGD.
Cellular componentGO:0005624membrane fractionIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0016579protein deubiquitinationIGI:SGD.
Biological processGO:0043328protein targeting to vacuole during ubiquit...IMP:SGD.
Biological processGO:0007584response to nutrientIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length844
Molecular weight97336

	---------+----------+----------+----------+----------+
MKPYLFDLKL KDTEKLDWKK GLSSYLKKSY GSSQWRTFYD EKATSELDHL   50
RNNANGELAP SSLSEQNLKY YSFLEHLYFR LGSKGSRLKM DFTWYDAEYS   100
SAQKGLKYTQ HTLAFEKSCT LFNIAVIFTQ IARENINEDY KNSIANLTKA   150
FSCFEYLSEN FLNSPSVDLQ SENTRFLANI CHAEAQELFV LKLLNDQISS   200
KQYTLISKLS RATCNLFQKC HDFMKEIDDD VAIYGEPKWK TTVTCKLHFY   250
KSLSAYYHGL HLEEENRVGE AIAFLDFSMQ QLISSLPFKT WLVEFIDFDG   300
FKETLEKKQK ELIKDNDFIY HESVPAVVQV DSIKALDAIK SPTWEKILEP   350
YMQDVANKYD SLYRGIIPLD VYEKESIYSE EKATLLRKQV EETETANLEY   400
SSFIEFTNLP RLLSDLEKQF SDGNIFSNTD TQGQLMRDQI QTWCKFIQTN   450
EFRDIEEQMN KIVFKRKQIL EILSALPNDQ KENVTKLKSS LVAASNSDEK   500
LFACVKPHIV EINLLNDNGK IWKKFDEFNR NTPPQPSLLD IDDTKNDKIL   550
ELLKQVKGHA EDLRTLKEER SRNLSELRDE INNDDITKLL IINKGKSDVE   600
LKDLFEVELE KFEPLSTRIE ATIYKQSSMI DDIKAKLDEI FHLSNFKDKS   650
SGEEKFLEDR KNFFDKLQEA VKSFSIFASD LPKGIEFYDS LFNMSRDLAE   700
RVRVAKQTED STANSPAPPL PPLDSKASVV GGPPLLPQKS AAFQSLSRQG   750
LNLGDQFQNL KISAGSDLPQ GPGIPPRTYE ASPYAATPTM AAPPVPPKQS   800
QEDMYDLRRR KAVENEEREL QENPTSFYNR PSVFDENMYS KYSS         844