SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBPT1
Systematic nameYLL015W
DescriptionBile pigment transporter 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2K1539        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP14772
SGD YLL015W
Gene Ontology (GO)
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...IDA:SGD.
Molecular functionGO:0015127bilirubin transporter activityIGI:SGD.
Molecular functionGO:0015086cadmium ion transporter activityIDA:SGD.
Biological processGO:0015723bilirubin transportIGI:SGD.
Biological processGO:0015691cadmium ion transportIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
645Phosphoserine.
885Phosphoserine.
927Phosphoserine.
931Phosphoserine.
934Phosphothreonine.

Sequence Information
Sequence length1559
Molecular weight176874

	---------+----------+----------+----------+----------+
MSSLEVVDGC PYGYRPYPDS GTNALNPCFI SVISAWQAVF FLLIGSYQLW   50
KLYKNNKVPP RFKNFPTLPS KINSRHLTHL TNVCFQSTLI ICELALVSQS   100
SDRVYPFILK KALYLNLLFN LGISLPTQYL AYFKSTFSMG NQLFYYMFQI   150
LLQLFLILQR YYHGSSNERL TVISGQTAMI LEVLLLFNSV AIFIYDLCIF   200
EPINELSEYY KKNGWYPPVH VLSYITFIWM NKLIVETYRN KKIKDPNQLP   250
LPPVDLNIKS ISKEFKANWE LEKWLNRNSL WRAIWKSFGR TISVAMLYET   300
TSDLLSVVQP QFLRIFIDGL NPETSSKYPP LNGVFIALTL FVISVVSVFL   350
TNQFYIGIFE AGLGIRGSLA SLVYQKSLRL TLAERNEKST GDILNLMSVD   400
VLRIQRFFEN AQTIIGAPIQ IIVVLTSLYW LLGKAVIGGL VTMAIMMPIN   450
AFLSRKVKKL SKTQMKYKDM RIKTITELLN AIKSIKLYAW EEPMMARLNH   500
VRNDMELKNF RKIGIVSNLI YFAWNCVPLM VTCSTFGLFS LFSDSPLSPA   550
IVFPSLSLFN ILNSAIYSVP SMINTIIETS VSMERLKSFL LSDEIDDSFI   600
ERIDPSADER ALPAIEMNNI TFLWKSKEVL TSSQSGDNLR TDEESIIGSS   650
QIALKNIDHF EAKRGDLVCV VGRVGAGKST FLKAILGQLP CMSGSRDSIP   700
PKLIIRSSSV AYCSQESWIM NASVRENILF GHKFDQDYYD LTIKACQLLP   750
DLKILPDGDE TLVGEKGISL SGGQKARLSL ARAVYSRADI YLLDDILSAV   800
DAEVSKNIIE YVLIGKTALL KNKTIILTTN TVSILKHSQM IYALENGEIV   850
EQGNYEDVMN RKNNTSKLKK LLEEFDSPID NGNESDVQTE HRSESEVDEP   900
LQLKVTESET EDEVVTESEL ELIKANSRRA SLATLRPRPF VGAQLDSVKK   950
TAQKAEKTEV GRVKTKIYLA YIKACGVLGV VLFFLFMILT RVFDLAENFW   1000
LKYWSESNEK NGSNERVWMF VGVYSLIGVA SAAFNNLRSI MMLLYCSIRG   1050
SKKLHESMAK SVIRSPMTFF ETTPVGRIIN RFSSDMDAVD SNLQYIFSFF   1100
FKSILTYLVT VILVGYNMPW FLVFNMFLVV IYIYYQTFYI VLSRELKRLI   1150
SISYSPIMSL MSESLNGYSI IDAYDHFERF IYLNYEKIQY NVDFVFNFRS   1200
TNRWLSVRLQ TIGATIVLAT AILALATMNT KRQLSSGMVG LLMSYSLEVT   1250
GSLTWIVRTT VTIETNIVSV ERIVEYCELP PEAQSINPEK RPDENWPSKG   1300
GIEFKNYSTK YRENLDPVLN NINVKIEPCE KVGIVGRTGA GKSTLSLALF   1350
RILEPTEGKI IIDGIDISDI GLFDLRSHLA IIPQDAQAFE GTVKTNLDPF   1400
NRYSEDELKR AVEQAHLKPH LEKMLHSKPR GDDSNEEDGN VNDILDVKIN   1450
ENGSNLSVGQ RQLLCLARAL LNRSKILVLD EATASVDMET DKIIQDTIRR   1500
EFKDRTILTI AHRIDTVLDS DKIIVLDQGS VREFDSPSKL LSDKTSIFYS   1550
LCEKGGYLK                                                1559

	* Ubiquitinated lysine is denoted as red color.