SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBIO2
Systematic nameYGR286C
DescriptionBiotin synthase, mitochondrial

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP32451
SGD YGR286C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004076biotin synthase activityTAS:SGD.
Biological processGO:0009102biotin biosynthetic processTAS:SGD.
EC number 2.8.1.6

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length375
Molecular weight41884

	---------+----------+----------+----------+----------+
MMSTIYRHLS TARPALTKYA TNAAVKSTTA SSEASTLGAL QYALSLDEPS   50
HSWTKSQLKE IYHTPLLELT HAAQLQHRKW HDPTKVQLCT LMNIKSGGCS   100
EDCKYCAQSS RNDTGLKAEK MVKVDEVIKE AEEAKRNGST RFCLGAAWRD   150
MKGRKSAMKR IQEMVTKVND MGLETCVTLG MVDQDQAKQL KDAGLTAYNH   200
NIDTSREHYS KVITTRTYDD RLQTIKNVQE SGIKACTGGI LGLGESEDDH   250
IGFIYTLSNM SPHPESLPIN RLVAIKGTPM AEELADPKSK KLQFDEILRT   300
IATARIVMPK AIIRLAAGRY TMKETEQFVC FMAGCNSIFT GKKMLTTMCN   350
GWDEDKAMLA KWGLQPMEAF KYDRS                              375