SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBGL2
Systematic nameYGR282C
AliasSCW9
DescriptionGlucan 1,3-beta-glucosidase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP15703
SGD YGR282C
Gene Ontology (GO)
Cellular componentGO:0009277chitin- and beta-glucan-containing cell wallTAS:SGD.
Molecular functionGO:0004338glucan 1,3-beta-glucosidase activityTAS:SGD.
Biological processGO:0007047cell wall organization and biogenesisTAS:SGD.
EC number 3.2.1.58

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length313
Molecular weight34119

	---------+----------+----------+----------+----------+
MRFSTTLATA ATALFFTASQ VSAIGELAFN LGVKNNDGTC KSTSDYETEL   50
QALKSYTSTV KVYAASDCNT LQNLGPAAEA EGFTIFVGVW PTDDSHYAAE   100
KAALQTYLPK IKESTVAGFL VGSEALYRND LTASQLSDKI NDVRSVVADI   150
SDSDGKSYSG KQVGTVDSWN VLVAGYNSAV IEASDFVMAN AFSYWQGQTM   200
QNASYSFFDD IMQALQVIQS TKGSTDITFW VGETGWPTDG TNFESSYPSV   250
DNAKQFWKEG ICSMRAWGVN VIVFEAFDED WKPNTSGTSD VEKHWGVFTS   300
SDNLKYSLDC DFS                                           313