SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBFR1
Systematic nameYOR198C
DescriptionNuclear segregation protein BFR1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38934
SGD YOR198C
Gene Ontology (GO)
Cellular componentGO:0042175nuclear envelope-endoplasmic reticulum networkIDA:SGD.
Cellular componentGO:0005844polysomeIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003723RNA bindingIDA:SGD.
Biological processGO:0007126meiosisIMP:SGD.
Biological processGO:0016071mRNA metabolic processIPI:SGD.
Biological processGO:0007088regulation of mitosisIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
32Phosphothreonine.
260Phosphoserine.
336Phosphothreonine.

Sequence Information
Sequence length470
Molecular weight54639

	---------+----------+----------+----------+----------+
MSSQQHKFKR PDVSVRDKKL DTLNVQLKKI DTEIGLIRKQ IDQHQVNDTT   50
QQERKKLQDK NKEIIKIQAD LKTRRSNIHD SIKQLDAQIK RKNNQIEEKL   100
GKKAKFSSTA EAKQRINEIE ESIASGDLSL VQEKLLVKEM QSLNKLIKDL   150
VNIEPIRKSV DADKAKINQL KEELNGLNPK DVSNQFEENQ QKLNDIHSKT   200
QGVYDKRQTL FNKRAALYKK RDELYSQIRQ IRADFDNEFK SFRAKLDKER   250
LKREEEQRLS KLLEQKDVDM GKLQEKLTHA KIPAFTYEIG AIENSLLVLD   300
PTYVKPKKNI LPDLSSNALE TKPARKVVAD DLVLVTPKKD DFVNVAPSKS   350
KKYKKKNQQK NTENEQPASI FNKVDGKFTL EPTLIATLAE LDVTVPINSD   400
DVKITVEQLK KKHEELLSKQ EEQTKQNIES VEKEIEKLNL DYSNKEQQVK   450
KELEEKRLKE QEESEKDKEN                                     470