SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameBEM2
Systematic nameYER155C
AliasIPL2, SUP9
DescriptionGTPase-activating protein BEM2/IPL2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP39960
SGD YER155C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0005100Rho GTPase activator activityIDA:SGD.
Molecular functionGO:0004871signal transducer activityIPI:SGD.
Biological processGO:0030036actin cytoskeleton organization and biogenesisIGI:SGD.
Biological processGO:0007117budding cell bud growthIPI:SGD.
Biological processGO:0007047cell wall organization and biogenesisIPI:SGD.
Biological processGO:0030468establishment of cell polarity (sensu Fungi)IPI:SGD.
Biological processGO:0000226microtubule cytoskeleton organization and b...IGI:SGD.
Biological processGO:0007264small GTPase mediated signal transductionIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
283Phosphoserine.
920Phosphoserine.
1012Phosphoserine.
1016Phosphoserine.
1046Phosphoserine.
1054Phosphoserine.

Sequence Information
Sequence length2167
Molecular weight245430

	---------+----------+----------+----------+----------+
MKGLLWSKNR KSSTASASSS STSTSHKTTT ASTASSSSPS SSSQTIRNST   50
SGASPYMHSH HHHGQGHSHH RGEDNNRDKR KSSVFPPSKQ YTSTSSSQVN   100
LGMYHSDTNT RSSRSIASTL KDDSPSVCSE DEISNSSSQK SNAQDETPIA   150
YKKSAHSKDS LLPSRSSSLS PPQSRCSTGT TLEKSLNTSG ISNSSGTNNN   200
NSNNNNDNEQ KQRNVIHLNS ENYDTTVFKT GWVNKSHGQT VATNYNSSMT   250
APSSSSSSSS QNLRNDAYSR NRESRFYGND GSSLKNDDSS STTATNSGND   300
VASARSSMAI DPQMLVPDYR LYRAQLKGCV LNLYKSGLNS NIKFFDPTLP   350
ASNSSIANEN HQQKKQQTNN QAQAEALHQK QSFGQMGEPI TLDLKYLSEV   400
YPHPDLRQDS DGKIISGTIE SLCHTVLFYP GPKQSDVPNE KSLSKTHRAV   450
INLLLMFPLL DHFIKFLKVF NQFGLSFTKN KSRLTNNSTQ FYNISPAVDD   500
SMTQRLALTA KTILDVFPGF LLDEPMLKTI ISLLDTISLH NDEISNNLKI   550
KIANKHNELM KLTAFTRSLP MATSSTHELE IILDPSHFLS LDITTLADEV   600
HHINLKFDKV WAPKFDYSLL YDSKFINRRI VSLNPLVFNN DQNIHFLGRL   650
LISHLFPTNP EFSKKVTPKV RAELLDKWVQ IGCRFEHLGD MVSWLAVATI   700
ICSIPVLRSS SWKYVPDQSL KTIFKDWVPT IIQLERRQRT SKSTSSVFIL   750
APPNLDDDFT RANVISYFGD LLIHADDLPS DTKFKYLEKK INRTKNAFHK   800
WQQRLQAIDS TRHKTNSTEN VRDNDSPNNV VYQLWKFHLS QPPLNIEGIM   850
KLSVQHEPPI IDQKAYSTIG SQRSALVTGS YLPILFNELF PNYSLFPKNT   900
LVGAASDAKL PPPRSSARLS KSLSISEPIP IASNSHTMGS LTDDAMSSKN   950
DNNKVTGVGK IDGPVIKEMS SKQSNKQRLL KSVRDVFNID MDVFHISDEL   1000
VFKSVYDNDG KSRPASMVIE TPKRFSQHSS MLINNPATPN QKMRDSLDTT   1050
GRLSKTLENM DFFNNIGQVS DSLKESIIRV VLKSSSLEKI FDLLVLTSNI   1100
FSKLVDTKDL ENYYYHQRQR GHSTRGLSDD NIGLLDYAFV KLTMDNDIFT   1150
ETFFNTYKSF TTTTTVLENM AKRYVGAKSC SVSISKILDR SDDSKMKINE   1200
DTNLVSSSLY DQNFPVWDMK VTDDENINLI YMAKIQIGAA EAILHLVKNH   1250
YSDFTDDLCN NSTLLDIIKI MEQEVSTEWP TRIANSKLQK SLPENFVIET   1300
ENLLTTLTDL FHGIKSAYQK QLYRPIGVNR TQKRITDILN SFNTFSFTDL   1350
NNIIDDPSFS DDMIRSFQKL HSTNYEDILE WIYQLDNFIS KKFNLVSKKD   1400
WIVLFQELEL LSKESLVSFF NYPLHFKSSK LINPGYLQLH EFEISNLFTW   1450
ISTLILKDDN GTESLFFEKL PQSIKLLIKL HTSLTTFFVM EISNVNKSSS   1500
ERLTTCKVIL QILNYIRWKN GSLDLFDSEE DESPHAICPH IPAFIETAIA   1550
HAIISPESRN YELSWIKASE KLSDPTKGTQ NLRSISNVLE KIDDIHIKRF   1600
IEIDDVFSKN CKNLCPCPGW FISRLLEISQ FVPNMSITNS KLINFDKRRF   1650
VNNIISNVLD LIPNEREFPL DIEMSDENPS KRTTFGRILF NNFEDVNKVY   1700
RKKTKKVSES EAISERFQEQ GVFNEILVNE IEKIKREARK LEVLLDQEKI   1750
LKNSAALHQA VPKKNRKSVI ISGTHSDNDH SYNINKNTGQ TPSLGSVMES   1800
NNSARNRRDS RASFSTNRSS VVSNSSHNGV SKKIGGFFRR PFSIGGFNTS   1850
SSNYSLNSIL SQEVSSNKSI LPSILPEVDS MQLHDLKPSY SLKTFEIKSI   1900
MEIINHRNIP AYYYAFKIVM QNGHEYLIQT ASSSDLTEWI KMIKASKRFS   1950
FHSKKYKGKT HNKIFGVPLE DVCERENTLI PTIVVKLLEE IELRGLDEVG   2000
LYRIPGSIGS INALKNAFDE EGATDNSFTL EDDRWFEVNA IAGCFKMYLR   2050
ELPDSLFSHA MVNDFTDLAI KYKAHAMVNE EYKRMMNELL QKLPTCYYQT   2100
LKRIVFHLNK VHQHVVNNKM DASNLAIVFS MSFINQEDLA NSMGSRLGAV   2150
QTILQDFIKN PNDYFKQ                                       2167