SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAVT3
Systematic nameYKL146W
DescriptionVacuolar amino acid transporter 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP36062
SGD YKL146W
Gene Ontology (GO)
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...IMP:SGD.
Molecular functionGO:0015186L-glutamine transporter activityIGI:SGD.
Molecular functionGO:0015188L-isoleucine transporter activityIGI:SGD.
Molecular functionGO:0005302L-tyrosine transporter activityIGI:SGD.
Biological processGO:0032974amino acid export from vacuoleIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
58Phosphoserine.
59Phosphoserine.
61Phosphothreonine.

Sequence Information
Sequence length692
Molecular weight75459

	---------+----------+----------+----------+----------+
MNGKEVSSGS GRTQSNNNKK NNNGGSTGIS HASGSPLTDG NGGNSNGNSR   50
SRSRSRKSSG TTGGLLKKPP LLVNNEAVHA SVPDASHTSC NNGTLEVSIN   100
NPEPHVVDAV ARHLIRNPSN SLQLQGGDIT RDLYKWTNDH PSSPSQYQYP   150
SQPALSTSIP SQAPSFSNRK RSMSFSAASI ASSSHLNNNS EANGNPLAAI   200
GLAPAPMTHE EIRAPGGFRR SFIIQKRRKH NVDAPIPNFF TRNFIEFLTL   250
YGHFAGEDLS EEEEEEEETE EEPEEEALET ESTQLVSREH GRHPHKSSTV   300
KAVLLLLKSF VGTGVLFLPK AFHNGGWGFS ALCLLSCALI SYGCFVSLIT   350
TKDKVGVDGY GDMGRILYGP KMKFAILSSI ALSQIGFSAA YTVFTATNLQ   400
VFSENFFHLK PGSISLATYI FAQVLIFVPL SLTRNIAKLS GTALIADLFI   450
LLGLVYVYVY SIYYIAVNGV ASDTMLMFNK ADWSLFIGTA IFTFEGIGLL   500
IPIQESMKHP KHFRPSLSAV MCIVAVIFIS CGLLCYAAFG SDVKTVVLLN   550
FPQDTSYTLT VQLLYALAIL LSTPLQLFPA IRILENWTFP SNASGKYNPK   600
VKWLKNYFRC AIVVLTSILA WVGANDLDKF VSLVGSFACI PLIYIYPPLL   650
HYKASILSGT SRARLLLDLI VIVFGVAVMA YTSWQTIKMW SQ           692