SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAVT1
Systematic nameYJR001W
DescriptionVacuolar amino acid transporter 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP47082
SGD YJR001W
Gene Ontology (GO)
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...IMP:SGD.
Molecular functionGO:0015186L-glutamine transporter activityIMP:SGD.
Molecular functionGO:0015188L-isoleucine transporter activityIMP:SGD.
Molecular functionGO:0005302L-tyrosine transporter activityIMP:SGD.
Biological processGO:0006865amino acid transportIMP:SGD.
Biological processGO:0007034vacuolar transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
181Phosphothreonine.
187Phosphoserine.

Sequence Information
Sequence length602
Molecular weight65346

	---------+----------+----------+----------+----------+
MPEQEPLSPN GRKRSEVHYI SIPLNRGSAF SPDDSVSQFQ SDGFMTRRQS   50
ILDHPVGSFK GVNSLSRFAT SLRRANSFRN IELNADNERS FFKESNDETY   100
DPDTLAPALD GRRLSVTLNN AGRPRITNLA NNDRVSTASM AIHDDDYGSI   150
QNSTIGDSGS ILRPTASLTE MMSGGAGRRF TNNDMDSIVV KRVEGVDGKV   200
VTLLAGQSTA PQTIFNSINV LIGIGLLALP LGLKYAGWVI GLTMLAIFAL   250
ATFCTAELLS RCLDTDPTLI SYADLGYAAF GTKGRALISA LFTLDLLGSG   300
VSLVILFGDS LNALFPQYST TFFKIVSFFI VTPPVFIPLS VLSNISLLGI   350
LSTTGTVLVI CCCGLYKSSS PGSLVNPMET SMWPIDLKHL CLSIGLLSAC   400
WGGHAVFPNL KTDMRHPDKF KDCLKTTYKI TSVTDIGTAV IGFLMFGNLV   450
KDEITKNVLL TEGYPKFVYG LISALMTIIP IAKTPLNARP IVSVLDVLMN   500
VQHIDEAASA IKRRAAKGLQ VFNRIFINVV FVLIAINFPE FDKIIAFLGA   550
GLCFTICLIL PCWFYLRLCK TTIKPWERVA CHVTICISVV LSTLGVGAAI   600
IS                                                       602