SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAST2
Systematic nameYER101C
DescriptionProtein AST2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP39945
SGD YER101C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length430
Molecular weight48371

	---------+----------+----------+----------+----------+
MAEKILENKD PKLEAMTVDH EVSAPKPIPV DEPTLTRVAR PLRHVRHIPV   50
KSLVFHSKHG PITFSYENKI KLPISKNKLV VQVNYVGLNP VDMKIRNGYT   100
KPIYGEAGIG REYSGVITHV GDNLTNRWNV GDDVYGIYYH PKLAIGALQS   150
SLLIDPRVDP ILMRPKNTLS PEKAAGSLFC LGTALNLLAQ LKEKDQLNTE   200
SNVLINGGTS SVGMFAIQLL KRYYKVSKKL VVVTSGNGAA VLSEHFPDLK   250
DEIIFINYLS CRGKSSKPLR RMLDTGKVVD YDDFNTLKET EDYTQGKFNV   300
VLDFIGGYDI LSHSSSLIHA KGAYITTVGD YVGNYKKDVF DSWDNPSANA   350
RKMFGSMLWS YDYSHFYFDP NIKIIPKKND WIHECGKLLN EGVVDCVVDK   400
VYSWKNFKEA FSYMATQRAQ GKLIMKVEGF                          430