SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameASN2
Systematic nameYGR124W
DescriptionAsparagine synthetase [glutamine-hydrolyzing] 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP49090
SGD YGR124W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0004066asparagine synthase (glutamine-hydrolyzing)...IMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006529asparagine biosynthetic processTAS:SGD.
EC number 6.3.5.4

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
204Phosphoserine.
205Phosphothreonine.

Sequence Information
Sequence length572
Molecular weight64593

	---------+----------+----------+----------+----------+
MCGIFAAFKH EDIHNFKPKA LQLSKKIRHR GPDWSGNAVM NSTIFVHERL   50
AIVGLDSGAQ PITSADGEYM LGVNGEIYNH IQLREMCSDY KFQTFSDCEP   100
IIPLYLEHDI DAPKYLDGMF AFCLYDSKKD RIVAARDPIG VVTLYMGRSS   150
QSPETVYFAS ELKCLTDVCD SIISFPPGHV YDSETDKITR YFTPDWLDEK   200
RIPSTPVDYH AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIAAIAARE   250
TEKANADANE DNNVDEKQLA GIDDQGHLHT SGWSRLHSFA IGLPNAPDLQ   300
AARKVAKFIG SIHHEHTFTL QEGLDALDDV IYHLETYDVT TIRASTPMFL   350
LSRKIKAQGV KMVLSGEGSD EIFGGYLYFA QAPSAAEFHT ESVQRVKNLH   400
LADCLRANKS TMAWGLEARV PFLDKDFLQL CMNIDPNEKM IKPKEGRIEK   450
YILRKAFDTT DEPDVKPYLP EEILWRQKEQ FSDGVGYSWI DGLRDTAERA   500
ISDAMFANPK ADWGDDIPTT KEAYWYRLKF DAWFPQKTAA DTVMRWIPKA   550
DWGCAEDPSG RYAKIHEKHV SA                                 572