SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameASH1
Systematic nameYKL185W
DescriptionTranscriptional regulatory protein ASH1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP34233
SGD YKL185W
Gene Ontology (GO)
Cellular componentGO:0005933cellular budIDA:SGD.
Cellular componentGO:0000508Rpd3L complexIDA:SGD.
Molecular functionGO:0016566specific transcriptional repressor activityIDA:SGD.
Biological processGO:0045892negative regulation of transcription, DNA-d...IDA:SGD.
Biological processGO:0007124pseudohyphal growthIMP:SGD.
Biological processGO:0007532regulation of transcription, mating-type sp...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
452Phosphoserine.

Sequence Information
Sequence length588
Molecular weight65685

	---------+----------+----------+----------+----------+
MSSLYIKTPL HALSAGPDSH ANSSYYDNLL LPSFSNLSSN ISRNNITTDN   50
NINSASPRKY SFHSLNVSPI LSPISLANEI LGKKSNTAPA SPHHMDYNPI   100
SSLTPGNSPE FNKASLSQIS FTNPLNYGSG LGFSSNSQPR LPLLDRLSSV   150
SLSKRPERPQ QSLPSLRHLQ LLPSPLLQEN AARFPDTSKR TSNWKTDLTH   200
WCKDTNYQDY VKIREEVAHF KPLSIPNLTN NQNNDSFNYG KELESTRSSK   250
FHSPSKESFD RTKLIPSILE AKDQFKDLSN NAWSITPPVT PPMSPPTNRT   300
MERTTLRGVE ASFFEGKSSN NDSIFNPIIS EKLVQEVKHQ RQLRGNSFPM   350
PNASHKKTNS FKALQIKKLL ANRDILSNNS KSNVRKPSKN KISKQASNVF   400
GNTARQLVMK LDNASYSSVS ASSSPSPSTP TKSGKMRSRS SSPVRPKAYT   450
PSPRSPNYHR FALDSPPQSP RRSSNSSITK KGSRRSSGSS PTRHTTRVCV   500
SCHSSDSPCW RPSWSPRKQD QLCNSCGLRY KKTHTRCLND LCRKIPTKGE   550
INIMKSNGID KEFVPERNCE IEGYRCLFCN YITETVEN                588