SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameARO4
Systematic nameYBR249C
DescriptionPhospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32449
SGD YBR249C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:00038493-deoxy-7-phosphoheptulonate synthase activityIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
EC number 2.5.1.54

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length370
Molecular weight39749

	---------+----------+----------+----------+----------+
MSESPMFAAN GMPKVNQGAE EDVRILGYDP LASPALLQVQ IPATPTSLET   50
AKRGRREAID IITGKDDRVL VIVGPCSIHD LEAAQEYALR LKKLSDELKG   100
DLSIIMRAYL EKPRTTVGWK GLINDPDVNN TFNINKGLQS ARQLFVNLTN   150
IGLPIGSEML DTISPQYLAD LVSFGAIGAR TTESQLHREL ASGLSFPVGF   200
KNGTDGTLNV AVDACQAAAH SHHFMGVTKH GVAAITTTKG NEHCFVILRG   250
GKKGTNYDAK SVAEAKAQLP AGSNGLMIDY SHGNSNKDFR NQPKVNDVVC   300
EQIANGENAI TGVMIESNIN EGNQGIPAEG KAGLKYGVSI TDACIGWETT   350
EDVLRKLAAA VRQRREVNKK                                     370